163,584 results

1. pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

   Plasmid

   #236251

   Purpose

   Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Inserts

   tTS/rtTA
   cymR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   rTS and rtTA_M2 linked by T2A

   Promoter

   CMV

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #71236

   Purpose

   Express sgRNA and dCas9-KRAB from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

   Inserts

   humanized dCas9-KRAB T2A Puro
   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3 Basic Vector

   Plasmid

   #212936

   Purpose

   Firefly luciferase vector for investigating regions controlling transcription

   Depositor

   Debrya Groskreutz

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. scramble shRNA

   Plasmid

   #1864

   Purpose

   3rd gen lentiviral negative control vector containing scrambled shRNA

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   scramble
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE6b

    Plasmid

    #207852

    Purpose

    Mammalian expression of PE6b prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6b
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-VSVG

    Plasmid

    #64084

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSGNluc

    Plasmid

    #186705

    Purpose

    A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

    Depositor

    Maria Leptin, Baubak Bajoghli

    Article

    Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

    Inserts

    GFP
    luciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-CMV-gagpol

    Plasmid

    #35614

    Depositor

    Patrick Salmon

    Insert

    MLV gag and pol genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-TRIM28

    Plasmid

    #124960

    Purpose

    Mammalian expression of Human TRIM28

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCold TDP43 WT

    Plasmid

    #27451

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa, GST, and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti CMV Blast DEST (706-1)

    Plasmid

    #17451

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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