169,603 results

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qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Lamin A/C

Plasmid

#246875

Purpose

Expression of mScarlet3-S2 fused with Lamin A/C. This construct is used for imaging the nuclear lamina.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Lamin A/C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFluc

Plasmid

#26156

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Expression

Bacterial

Mutation

Firefly luciferase gene codon optimised for mycob…

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Blast

Plasmid

#207709

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

Plasmid

#208048

Purpose

Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

BirAopt

Use

Lentiviral

Mutation

BirAopt is human-codon-optimized BirA (PMID 15707…

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVHA SUZ12

Plasmid

#24232

Purpose

Mammalian expression of human SUZ12 with HA tag

Depositor

Kristian Helin

Article

Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

Insert

SUZ12 (SUZ12 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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hSYN-mito-dsRED2

Plasmid

#173069

Purpose

mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPS- SIV-MAC251 vpx

Plasmid

#115810

Purpose

Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251 Vpx

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC7A11

Plasmid

#197993

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SQSTM1-WT

Plasmid

#194582

Purpose

Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SQSTM1 (SQSTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag mTOR wt

Plasmid

#26603

Purpose

Mammalian expression of human flag-tagged mTor

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pBP-T7_RBS-His6-Thrombin

Plasmid

#72979

Purpose

T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7+RBS+His6+thrombin

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1)

Plasmid

#17452

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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TFORF1917

Plasmid

#143554

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX2 (RUNX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PhoDer

Plasmid

#188473

Purpose

Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

Depositor

Gary Mo

Article

Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

Insert

PhoDer (GSDMD Synthetic, Human)

Expression

Mammalian

Promoter

CMV IE

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-60 Grx1-roGFP2-His

Plasmid

#64799

Purpose

Bacterial expression of His-tagged Grx1-roGFP2

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Tags

His and roGFP2

Expression

Bacterial

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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A24M04 Ig kappa light chain-pFEEKW

Plasmid

#234810

Purpose

Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

Depositor

Susan Pierce

Article

Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

A24M04 Igkappa light chain (IGKC, IGK Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EEK promoter

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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