19,619 results

1. pCW-Flag-CtIP-T847E

   Plasmid

   #71110

   Purpose

   mammalian expession of Flag-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. APEX2-NLS

   Plasmid

   #124617

   Purpose

   proximity biotinylation

   Depositor

   Alice Ting

   Article

   Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

   Insert

   APEX2-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TrkB Acceptor

   Plasmid

   #83953

   Purpose

   FRET acceptor for TrkB mEGFP

   Depositor

   Ryohei Yasuda

   Article

   Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

   Insert

   Phospholipase C gamma 1 (Plcg1 Mouse)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-mCherry-LOV2

   Plasmid

   #81041

   Purpose

   Mammalian or insect expression for wt LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV2
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV, p10

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmGFP-ADAR1-p150

   Plasmid

   #117927

   Purpose

   ADAR1-p150 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p150 (ADAR Human)
   

   Tags

   myc-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-PGK-ABI-dCas9-P2A-mcherry

   Plasmid

   #121513

   Purpose

   expression of AB1-fused-dcas9 plasmids

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   AB1 and P2A-mcherry

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FGFR2 3xFlag

   Plasmid

   #110063

   Purpose

   FGFR2 expression in mammalian cells (JCOPCO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-N-Flag-SREBP1

    Plasmid

    #134283

    Purpose

    Expresses Flag-tagged SREBP1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9-2A-Cre

    Plasmid

    #68468

    Purpose

    co-expressing Cas9 and Cre recombinase

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    Cas9 and Cre recombinase
    

    Use

    CRISPR

    Tags

    P2A-Cre-Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAPB02

    Plasmid

    #65628

    Purpose

    A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

    Depositor

    Pei-yuan Qian

    Article

    Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPLICS Po-ER Short P2A

    Plasmid

    #164114

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-BTK-mCherry

    Plasmid

    #50043

    Purpose

    Expression of BTK-mCherry in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BTK (Btk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGreen_dualluc_ORF_sensor

    Plasmid

    #55207

    Purpose

    Dual-luciferase reporter for microRNA targeting in plants

    Depositor

    Michael Axtell

    Article

    Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Luciferase; Plant transient expression

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. hCD4-mOrange

    Plasmid

    #110192

    Purpose

    Encodes for human CD4 coding sequence tagged with the mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCD4 (CD4 Human)
    

    Tags

    mOrange

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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