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20,196 results

Showing: 3081 - 3120 of 20196 results

  4. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  6. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  9. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  19. pRS303-PADH1-AFB2

    Plasmid
    #99530
    Purpose
    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)
    Depositor
    Helle Ulrich
    Insert
    AFB2 (AFB2 Mustard Weed)
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99530-plasmid-map-sequence-id-190861

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  23. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. β€˜GianCreg’ is an abbreviation for giantin C-terminal region. β€˜=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    GianCreg (GOLGB1 Human)
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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  25. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  31. pSG5 Large T

    Plasmid
    #9053
    Depositor
    William Hahn
    Insert
    large T antigen (SV40gp6 simian virus)
    Expression
    Mammalian
    Available Since
    Jan. 5, 2006
    Availability
    Academic Institutions and Nonprofits only
    9053-plasmid-map-sequence-id-244756

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  34. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  36. p-dCas9-HDAC1-Hygro

    Plasmid
    #104409
    Purpose
    transient expression of dCas9-HDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    HDAC1 (HDAC1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104409-plasmid-map-sequence-id-204471

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  37. pCDH-CB

    Plasmid
    #72267
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72267-plasmid-map-sequence-id-131747

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Showing: 3081 - 3120 of 20196 results