166,806 results

1. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM184_30x147bp_Widom601

   Plasmid

   #240634

   Purpose

   30x 147-bp Widom 601 nucleosome positioning sequence

   Depositor

   Emma Morrison

   Article

   Hammonds et al bioRxiv [Preprint]. 2025 Apr 10:2025.04.

   Insert

   30x 147-bp Widom 601
   

   Use

   Dna amplification

   Available Since

   Sept. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-TBC1D3B

   Plasmid

   #116798

   Purpose

   Lentiviral expression of TBC1D3B

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   TBC1D3B (TBC1D3B Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNX32-Dest

   Plasmid

   #105083

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Tags

   NubG

   Expression

   Yeast

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/CIP2A(1-905) WT V5 His

   Plasmid

   #119287

   Purpose

   CIP2A WT V5 overexpression

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Tags

   V5 His

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-mTagBFP2

   Plasmid

   #122373

   Purpose

   Expresses mTagBFP2 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTEF2

   Plasmid

   #43971

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Promoter

   TEF

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLC242-GFP-metap2

   Plasmid

   #100518

   Purpose

   Expresses GFP tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   Metap2 (METAP2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSD141B

   Plasmid

   #218139

   Purpose

   Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD56-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ankG-mCherry

    Plasmid

    #42566

    Depositor

    Benedicte Dargent

    Article

    Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    ankyrin G (Ank3 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3HA-ERHBD-Hoxb8

    Plasmid

    #212932

    Purpose

    Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

    Depositor

    Hans Haecker

    Article

    Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

    Insert

    3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Triple HA tag

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-FRT/T0-Flag-PALB2

    Plasmid

    #71114

    Purpose

    mammalian expession of FLAG-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Fos-CreERT2

    Plasmid

    #194643

    Purpose

    Expresses Cre-ERT2 under the control of c-Fos promoter

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    Fos-CreERT2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK348 (BSD-P2A-mAID-mClover)

    Plasmid

    #121182

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

    Plasmid

    #204808

    Purpose

    Bacterial co-expression of Dengue2 NS2B and NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue2 NS2B (POLY Dengue2)
    

    Expression

    Bacterial

    Available Since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mRFP1-N1

    Plasmid

    #54635

    Purpose

    Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

    Depositor

    Robert Campbell, Michael Davidson, Roger Tsien

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

    Type

    Empty backbone

    Tags

    mRFP1

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hsyn-GRAB_Ado1.0

    Plasmid

    #153285

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJ4M_hnRNPA2_FL_WT

    Plasmid

    #139109

    Purpose

    Express FL hnRNPA2 with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEG BacMam C term GFP His8

    Plasmid

    #160681

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    His8 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. ssFv_lenti_wtATMSPARK2

    Plasmid

    #195249

    Purpose

    express wtATMSPARK2 in a lenti vector

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    ATMSPARK2 sensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    ssfv

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGPD2

    Plasmid

    #43972

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    GPD

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. CMV-enAsCas12f

    Plasmid

    #204637

    Purpose

    Expression of enAsCas12f in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

    Plasmid

    #127872

    Purpose

    Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pNP3386 [For use in genome insertion and off-target assessment]

    Plasmid

    #247258

    Purpose

    Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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