We narrowed to 18,831 results for: Tec

1. pHCKan-yibDp-GFPuv

   Plasmid

   #127078

   Purpose

   Low phosphate inducible yibD gene promoter expressing GFPuv reporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pR26-CAG-H2B-miRFP703

   Plasmid

   #112853

   Purpose

   Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   H2B-miRFP703
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPomyces-Sth1Cas9

   Plasmid

   #129552

   Purpose

   Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   Sth1Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_BE4max_3xHA

   Plasmid

   #112096

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   BE4max_3xHA
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPomyces-FnCpf1

   Plasmid

   #129554

   Purpose

   Contains codon-optimized FnCpf1 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   FnCpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYL322d1/Vn-C

   Plasmid

   #183154

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1P shSOD2

   Plasmid

   #102976

   Purpose

   Suppression of SOD2

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shSOD2 (SOD2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RNA motif plasmid EDEN15

   Plasmid

   #107252

   Purpose

   EDEN15 RNA motif

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   EDEN15 motif
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mStrawberry-N1

   Plasmid

   #54644

   Purpose

   Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

   Depositor

   Nathan Shaner, Michael Davidson, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG3 N

    Plasmid

    #132941

    Purpose

    MLV packaging plasmid for N-tropic MLV gag-pol expression

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    N-tropic MLV gag-pol
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSFVCs-lacZ

    Plasmid

    #92076

    Purpose

    This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

    Depositor

    Henrik Garoff

    Article

    Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

    Insert

    C-lacZ
    

    Use

    C-lacz expression plasmid, for run-off transcript…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. BFP dest clone

    Plasmid

    #71825

    Purpose

    Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT423

    Plasmid

    #139382

    Purpose

    Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Constitutively expressed dCas9, LacI and TetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P1

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB1620 hU6-BpiI-Cas13bt3-DR

    Plasmid

    #176315

    Purpose

    hU6-BpiI-Cas13bt3-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt3 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. dTomato_LD 0_TEV

    Plasmid

    #58872

    Purpose

    MESA protease chain with dTomato ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with dTomato ectodomain and TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19/N-mChe

    Plasmid

    #183159

    Purpose

    Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Septin 11 isoform II-EGFP

    Plasmid

    #120316

    Purpose

    Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Septin 11 isoform II

    Plasmid

    #120318

    Purpose

    Septin 11 isoform II expression (mCherry-tag at the N-terminus)

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mSA-phi29

    Plasmid

    #210815

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    mSA-Phi29
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB2195

    Plasmid

    #67548

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

    Plasmid

    #55219

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Reporter-2

    Plasmid

    #180219

    Purpose

    Surrogate reporter plasmid for the assay of targeted RNA editing

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Insert

    Surrogate reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pORF1-2

    Plasmid

    #206024

    Purpose

    An expression vector for mouse LINE1 ORF1-2

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    ORF1-2 derived from mouse LINE1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCherry-FRB

    Plasmid

    #25920

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-GAVPO

    Plasmid

    #159923

    Purpose

    GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    GAVPO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDYU1G/γ-tubulin-mEGFP

    Plasmid

    #154301

    Purpose

    Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Insert

    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    actin15

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ABE-RA2.0

    Plasmid

    #208299

    Purpose

    Expresses ABE-RA2.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.0-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ABE-RA3.1

    Plasmid

    #208302

    Purpose

    Expresses ABE-RA3.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmRFP_a_tubulin_C1

    Plasmid

    #21040

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    pmRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pX330A_dCas9-1x7

    Plasmid

    #63601

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. DL015:CMV::iPAK4

    Plasmid

    #177880

    Purpose

    ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCpf1

    Plasmid

    #122185

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PlayBack-Ef1a-EcoR1

    Plasmid

    #203311

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. JME4599

    Plasmid

    #129660

    Purpose

    NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. DL016:CMV::eGFP-iPAK4

    Plasmid

    #177881

    Purpose

    eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSET FLIPE-600n

    Plasmid

    #13537

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-10His-StayGold

    Plasmid

    #211362

    Purpose

    Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    StayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge