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Showing: 351 - 400 of 12598 results

  1. pCAG-mTHC

    Plasmid
    #112620
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112620-plasmid-map-sequence-id-226488

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  6. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Insert
    RNMT (RNMT Human)
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112708-plasmid-map-sequence-id-217040

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  19. pARNTL-donor

    Plasmid
    #112384
    Purpose
    CRISPR donor plasmid to tag human transcription factor ARNTL with GFP
    Depositor
    Kevin White
    Insert
    ARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
    Use
    CRISPR
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112384-plasmid-map-sequence-id-229842

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  20. LUC45 (L/Sec/mutP)

    Plasmid
    #112148
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and SE…
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112148-plasmid-map-sequence-id-214874

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  21. pcDNA3.1-hRIPK1-Flag-S320A

    Plasmid
    #112491
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S320A
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112491-plasmid-map-sequence-id-214534

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  26. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Yingfeng An
    Article
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Tags
    ELP-tag
    Expression
    Bacterial
    Available Since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    112198-plasmid-map-sequence-id-218116

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  32. FFTTH--GFP-Amotl2

    Plasmid
    #112833
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112833-plasmid-map-sequence-id-217927

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  35. pORFE0005

    Plasmid
    #112073
    Purpose
    AtDeadCAS9 D10A H840A without stop codon module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112073-plasmid-map-sequence-id-217642

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  37. pQE80L TriCatcher-RGDSP-MMP

    Plasmid
    #112633
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-RGDSP-MMP
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available Since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112633-plasmid-map-sequence-id-222151

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  40. pORFE0011

    Plasmid
    #112075
    Purpose
    pcUBI promoter module Pro+5U
    Depositor
    Christian Chevalier
    Insert
    pcUBI promoter
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112075-plasmid-map-sequence-id-217625

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  41. pOP3SU

    Plasmid
    #112599
    Purpose
    Used for expression of protein in E. coli as N-terminal Sumo tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    Sumo
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112599-plasmid-map-sequence-id-220209

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  42. pExp-DsbA

    Plasmid
    #112562
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbA(AA20-208) and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112562-plasmid-map-sequence-id-217223

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  43. TAP-GFP-Amotl2

    Plasmid
    #112835
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    GFP and TAP
    Expression
    Mammalian
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112835-plasmid-map-sequence-id-217938

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  44. Aahn

    Plasmid
    #112158
    Purpose
    A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.
    Depositor
    Bradley Baker
    Article
    Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1
    Insert
    Aahn
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    112158-plasmid-map-sequence-id-220028

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  47. pARNTL.1.0-gDNA

    Plasmid
    #112480
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL
    Depositor
    Kevin White
    Insert
    ARNTL (BMAL1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112480-plasmid-map-sequence-id-230516

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  50. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112617-plasmid-map-sequence-id-224553

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Showing: 351 - 400 of 12598 results