We narrowed to 10,180 results for: yeast
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Plasmid#166077PurposePlasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeastDepositorInsertYBL055C (near PTC3) (YBL055C Budding Yeast)
UseCRISPRExpressionYeastPromoterTet-inducibleAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YNR071C
Plasmid#166081PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.DepositorInsertPromoter of YNR071C (YNR071C Budding Yeast)
UseCRISPRExpressionYeastPromoterTet-inducibleAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_SLA1
Plasmid#166075PurposePlasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeastDepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PDR12
Plasmid#166093PurposePlasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeastDepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH2
Plasmid#166082PurposePlasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeastDepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_LYS9
Plasmid#166085PurposePlasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeastDepositorAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAS9id_TRP
Plasmid#141254PurposeGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterGAL1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Peripherin/pAS2-1
Plasmid#98135PurposePeripherin (NM_006262) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertPeripherin (PRPH Human)
ExpressionYeastAvailable SinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTH341-SUP45(139-437)-GalBD
Plasmid#29741DepositorInsertSUP45 (SUP45 Budding Yeast)
TagsGal Activation domain and MycExpressionYeastMutationCodons 139-437 of the yeast SUP45 gene onlyAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH339-SUP45(1-138)-GalBD
Plasmid#29739DepositorInsertSUP45 (SUP45 Budding Yeast)
TagsGal Activation domain and MycExpressionYeastMutationCodons 1-138 of the yeast SUP45 gene onlyAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH342-SUP45(276-437)-GalBD
Plasmid#29742DepositorInsertSUP45 (SUP45 Budding Yeast)
TagsGal Activation domain and MycExpressionYeastMutationCodons 276-437 of the yeast SUP45 gene onlyAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_haPD-1
Plasmid#226716PurposePlasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)DepositorInsertHigh affinity PD-1 microbody
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_rPD-L1nb
Plasmid#226717PurposePlasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag, Histidine tag, and Mating factor alpha se…ExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FUS-FusionRed
Plasmid#188393PurposeCu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cellsDepositorAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-GW
Plasmid#61703PurposeGateway compatible Y2H vector - BDDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-GW
Plasmid#61702PurposeGateway compatible Y2H vector - ADDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT166_GalL_A3A-BE3
Plasmid#145116PurposeExpressing base editor A3A-BE3 in yeast cellsDepositorInsertA3A-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorExpressionYeastPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM0641
Plasmid#129006PurposeIntegration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT040
Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52Available SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
Plasmid#183150PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)DepositorInsertsLex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD
UseFor yeast genomic integrationAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
TagsV5 tagExpressionYeastPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWR76
Plasmid#202776Purposeepisomal fluorescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS426 Gal TDP43 GFP
Plasmid#27467DepositorAvailable SinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
Tagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-PEX3
Plasmid#51572PurposeConstitutively express peroxisome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsPEX3 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKanB alpha
Plasmid#223524PurposeUsed for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneExpressionYeastPromoterAOX1Available SinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVB3011
Plasmid#113389PurposeGalactose-inducible L-BC gag: cure killerDepositorInsertL-BC Gag
ExpressionYeastMutationPlease see depositor commentsAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM30
Plasmid#133673PurposeIntegration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1
Plasmid#39280DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable SinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP (F64L, S65T, V163A)-TRP1
Plasmid#53184Purposechromosomal tagging with stable GFPDepositorAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-PmCDA1
Plasmid#79615PurposeExpresses dCas9-PmCDA1 in yeast cellsDepositorInsertSpCas9
TagsPmCDA1ExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
ExpressionYeastAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3B
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS425ADS
Plasmid#20119DepositorInsertamorphadiene synthase
UseSynthetic BiologyExpressionYeastAvailable SinceMarch 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3rN_14*
Plasmid#98550Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC lambda (JCE590)
Plasmid#89775Purposeyeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminatorDepositorAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
mito-yHS1
Plasmid#159153PurposeMitochondrial labile heme reporterDepositorInsertmito-yHS1
TagsCOX4 pre-sequenceExpressionYeastPromoterGPDAvailable SinceSept. 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CDC10
Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only