164,577 results

1. MP6

   Plasmid

   #69669

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

   Plasmid

   #167935

   Purpose

   Inducible CRISPRi expression

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   KRAB-dCas9-DHFR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   tetON

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-NLS-HA-Bxb1

   Plasmid

   #51271

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   NLS-HA-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK18msB

   Plasmid

   #177839

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

   Depositor

   Christopher Johnson

   Article

   Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. c-myc-PT3EF1a

   Plasmid

   #92046

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Xin Chen

   Article

   Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

   Insert

   C-MYC (MYC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   July 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. v5 eVLP gag-pro-pol

    Plasmid

    #228466

    Purpose

    Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag-pro-pol(Q226P)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q226P

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. rtTA-N144

    Plasmid

    #66810

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    Reverse tetracycline-controlled transactivator
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid

    #44362

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Article

    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV; Adeno associated viral vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_Gal4UAS_IRES_mC_pGK_tBFP

    Plasmid

    #79123

    Purpose

    Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. peGFP C3-SPG20

    Plasmid

    #218578

    Purpose

    Expresses GFP-tagged human SPG20 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4-ApoE2

    Plasmid

    #87085

    Purpose

    Expresses ApoE2 in Mammalian cells

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    Apolipoprotein E2 (APOE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-evoCAST-Cas6

    Plasmid

    #234725

    Purpose

    Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseCas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-evoCAST-eCas7

    Plasmid

    #234726

    Purpose

    Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ePseCas7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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