Addgene

We narrowed to 8,793 results for: CAG

Showing: 2401 - 2430 of 8793 results

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pAAV[FLEX]-CAG-EGFP-miR-133-sponge

Plasmid

#235155

Purpose

Expresses a EGFP-based sponge for miR-133 in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/miR-133 sponge with 12x binding sites

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

Plasmid

#234724

Purpose

All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

Depositor

Kirill Martemyanov

Article

Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

Type

Empty backbone

Use

CRISPR

Tags

T2A-GFP

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV PHP.eB-CAG-Venus-P2A-Tau-RING

Plasmid

#233696

Purpose

AAV expression of Venus-P2A-Tau-RING from CAG promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus-P2A-Tau-RING

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-RLIM-IRES-mCherry

Plasmid

#232243

Purpose

Mammalian expression of RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

RLIM-GFP-IRES-mCherry (RLIM Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1[degron]-IRES-mCherry

Plasmid

#232240

Purpose

Mammalian expression of REX1[degron]-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

REX1 (aa 70-85)

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

Plasmid

#231208

Purpose

Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)

Use

Lentiviral

Tags

Expression

Mutation

L80F, R81D, L85V, I86V, I92F

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

Plasmid

#231205

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Tags

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Tags

Expression

Mutation

L147I, R148H, I203V, L290V

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

Plasmid

#218870

Purpose

Mammalian expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2(no bind)

Use

Tags

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L476/L479D

Plasmid

#210845

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L476/L479D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (450-517)-A452D/F453D

Plasmid

#210851

Purpose

Mammalian expression of ATG13 (450-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

A452D/F453D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-F509/F512D

Plasmid

#210847

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F509/F512D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

Plasmid

#210842

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L495/L499D

Plasmid

#210846

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L495/L499D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-F466D/F470D

Plasmid

#210844

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F466D/F470D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p254-RV-CAG-dio-mScarlet-T2A-Dach1-202

Plasmid

#204742

Purpose

Conditional expression of target gene Dach1 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Dach1

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFPnls barcode-210-SV40 polyA

Plasmid

#190876

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFPnls-barcode210

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

Plasmid

#188274

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX(T26R/K610R/K808R)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

Plasmid

#196874

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynGFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194726

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D908A

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

Plasmid

#188073

Purpose

To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 and ATG101 (ATG9A, ATG101 Human)

Use

Tags

GST tag

Expression

Mammalian

Mutation

codon-optimized ATG9 (828-839) and codon-optimize…

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

Plasmid

#172477

Purpose

sfGFP with a synthetic intron derived from the human beta-globin gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

Plasmid

#172478

Purpose

sfGFP with a synthetic intron from the pCI expression vector (Promega)

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

Plasmid

#172475

Purpose

sfGFP with a synthetic intron derived from the mouse Ighe gene

Depositor

Mario Capecchi

Insert

sfGFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge