We narrowed to 18,831 results for: Tec

1. 3xHA-BioID_pAS28

   Plasmid

   #118223

   Purpose

   expresses 3xHA-tagged BioID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   BioID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   R118G

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Tet-O-FUW-Nkx6.1

   Plasmid

   #45846

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Nkx6.1 (Nkx6-1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-ON

   Available Since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB6574

   Plasmid

   #106132

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

   Plasmid

   #55216

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PBS Actb-2A-TIR1-GFP

   Plasmid

   #113832

   Purpose

   targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   osTIR1-GFP (NEWENTRY Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tet-O-FUW-Zfp536

   Plasmid

   #45845

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

   Insert

   Zfp536 (Zfp536 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-CTLA4-mgSrtA

   Plasmid

   #125794

   Purpose

   for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CTLA4-mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDisplay-ZHER-mgSrtA

    Plasmid

    #125793

    Purpose

    for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    ZHER-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIp128-P30-His-POL30

    Plasmid

    #99542

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDisplay FLIPE-600n

    Plasmid

    #13545

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PSuperPuroRetro-1660(hPot1)

    Plasmid

    #11135

    Depositor

    Christopher Counter

    Article

    Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

    Insert

    RNAi against human Protection of Telomeres 1 (POT1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

14. JME4390

    Plasmid

    #129656

    Purpose

    LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTK297

    Plasmid

    #59566

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak mKate-G8-L7Ae 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTK299

    Plasmid

    #59568

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFPNL1

    Plasmid

    #61651

    Purpose

    Gateway vector for generating nucleus-targeted fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pIM1708-surT-PsurP-agaN-pNW33N

    Plasmid

    #44009

    Depositor

    Ichiro Matsumura

    Article

    Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

    Insert

    surT-PsurP-agaN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted EcoRI site (synonymous change, C to T at …

    Promoter

    Available Since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LBJJ382

    Plasmid

    #117518

    Purpose

    Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGT5439

    Plasmid

    #192351

    Purpose

    L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tOCS_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCellFree_G03 CYP2A13

    Plasmid

    #67103

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2A13 (CYP2A13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pENTR-L1-PGK-TKBsd-L2

    Plasmid

    #141009

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKBsd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p1.1

    Plasmid

    #162737

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDM_noro_A2_lacZA

    Plasmid

    #118819

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YEp112-CUP1-His-Smt3

    Plasmid

    #99537

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLH-sgRNA1-PP7-boxB

    Plasmid

    #75393

    Purpose

    sgRNA1-PP7-boxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7-boxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBN449

    Plasmid

    #129555

    Purpose

    GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans

    Depositor

    Peter Askjaer

    Article

    Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

    Insert

    15xUAS-∆pes-10-Flp-SL2
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    UAS-∆pes-10

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSBY2_ligD(FnCpf1)

    Plasmid

    #104623

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    crRNA to ligD
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pj23119

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. DL034:cFos::HaloTag-iPAK4

    Plasmid

    #177884

    Purpose

    HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUE1-TSP-UL32

    Plasmid

    #162658

    Purpose

    Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCellFree_G03 WWP1

    Plasmid

    #67125

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pColE1_sgRNA_T2_AmpR

    Plasmid

    #119159

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pColE1_sgRNA_T3_AmpR

    Plasmid

    #119160

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pColE1_sgRNA_T1_AmpR

    Plasmid

    #119158

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC-DEST-hSOX2-EGFP-PuroTK

    Plasmid

    #141026

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV5M/rDlg1

    Plasmid

    #28259

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Dlg1 I3
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only