Skip to main content
Addgene
Showing: 3481 - 3520 of 158338 results

  3. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

    Enlarge

  5. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Article
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

    Enlarge

  9. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

    Enlarge

  12. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41390-plasmid-map-sequence-id-352470

    Enlarge

  15. pLXSN16E6E7

    Plasmid
    #52394
    Purpose
    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.
    Depositor
    Denise Galloway
    Article
    Halbert et al J Virol. 1991 Jan;65(1):473-8.
    Insert
    HPV16 E6E7
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    52394-plasmid-map-sequence-id-371138

    Enlarge

  16. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

    Enlarge

  23. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

    Enlarge

  25. pAAV-hSyn-DIO-EGFP

    Plasmid
    #50457
    Purpose
    Double floxed EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50457-plasmid-map-sequence-id-162265

    Enlarge

  26. pAAVone-AAV9-CMV-eGFP

    Plasmid
    #230928
    Purpose
    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.
    Depositor
    Qizhao Wang
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230928-plasmid-map-sequence-id-458358

    Enlarge

  27. pRMC2

    Plasmid
    #68940
    Purpose
    tetracycline-inducible expression vector for Staphylococcus aureus
    Depositor
    Tim Foster
    Article
    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tet inducible e. coli/staphylococcus shuttle vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    68940-plasmid-map-sequence-id-171245

    Enlarge

  29. iC13 PB-TO-oNgn2-BFP-puro

    Plasmid
    #204733
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Insert
    Optimized neurogenin-2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    204733-plasmid-map-sequence-id-403381

    Enlarge

  32. pCasPA

    Plasmid
    #113347
    Purpose
    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa
    Depositor
    Quanjiang Ji
    Article
    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    113347-plasmid-map-sequence-id-237850

    Enlarge

  37. M13-kmR

    Plasmid
    #206861
    Purpose
    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
    Depositor
    Irene Chen
    Article
    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371
    Insert
    Kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    206861-plasmid-map-sequence-id-412778

    Enlarge

Showing: 3481 - 3520 of 158338 results