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Caenorhabditis elegans

1,859 results

Showing: 401 - 450 of 1859 results

  5. pHJL2

    Plasmid
    #199377
    Purpose
    Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::SL2::mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199377-plasmid-map-sequence-id-390940

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  9. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  22. pAS1-rab-3P::his-24::CyOFP

    Plasmid
    #124347
    Purpose
    Nuclear Localized Pan-Neuronal CyOFP
    Depositor
    Andrew Leifer
    Insert
    rab-3, CyOFP (rab-3 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124347-plasmid-map-sequence-id-241978

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  28. YW249_Pzipt17-loxP-H2BGFP-miniMOS

    Plasmid
    #196063
    Purpose
    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.
    Depositor
    Wenxing Yang
    Article
    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77
    Inserts
    H2B::GFP
    NeoR
    Minimal Mos1
    zipt17p::zipt17::zipt17 3UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196063-plasmid-map-sequence-id-383077

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  29. pJJS010

    Plasmid
    #188333
    Purpose
    PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mKate2::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188333-plasmid-map-sequence-id-370755

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  30. pJJS004

    Plasmid
    #188327
    Purpose
    PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mCherry::mIAA7
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188327-plasmid-map-sequence-id-370750

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  31. pJH2523

    Plasmid
    #191358
    Purpose
    Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    GCaMP3
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Punc-25
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191358-plasmid-map-sequence-id-377515

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  32. pIN4_tsp-6_mScarlet

    Plasmid
    #182935
    Purpose
    Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)
    Depositor
    Maureen Barr
    Article
    Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6
    Insert
    Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
    Use
    Tags
    flexible linker (GGGGS)x3 and mScarlet tag
    Expression
    Worm
    Mutation
    Promoter
    tsp-6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    182935-plasmid-map-sequence-id-368447

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  34. pMLS791

    Plasmid
    #188889
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Use
    Tags
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Promoter
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188889-plasmid-map-sequence-id-370713

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  36. pSiM4

    Plasmid
    #199383
    Purpose
    Encodes dual cistronic membrane localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    jGCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199383-plasmid-map-sequence-id-391021

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  37. pNMSB99

    Plasmid
    #199308
    Purpose
    MosSCI insertion of myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    myo-3p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…
    Promoter
    myo-3
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199308-plasmid-map-sequence-id-404762

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  38. pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid
    #159872
    Purpose
    Three-fragment Gateway compatible protein tag for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159872-plasmid-map-sequence-id-318863

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  44. pSAH66 - [BAGp | gfp | tbb-2 UTR]

    Plasmid
    #200340
    Purpose
    gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001536, flp-17
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    200340-plasmid-map-sequence-id-418120

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  45. pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200339
    Purpose
    mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001536, flp-17
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    200339-plasmid-map-sequence-id-409767

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  47. pLT 652

    Plasmid
    #59997
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59997-plasmid-map-sequence-id-110770

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  48. pSA120

    Plasmid
    #59789
    Purpose
    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59789-plasmid-map-sequence-id-95470

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  49. pMS051

    Plasmid
    #137025
    Purpose
    SapTrap mScarlet-I-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mScarlet
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137025-plasmid-map-sequence-id-274042

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Showing: 401 - 450 of 1859 results