We narrowed to 37,476 results for: Ran;
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Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
TagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
UseLentiviralExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGFP
Plasmid#14757DepositorHas ServiceCloning Grade DNAInsertmembrane bound form of EGFP
TagsPalmitoylation signalExpressionMammalianPromoterpCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab5
Plasmid#14437PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.DepositorAvailable SinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
SBE4-Luc
Plasmid#16495PurposeLuciferase reporter containing four copies of the Smad binding siteDepositorInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
Tags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
HRE-luciferase
Plasmid#26731PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.DepositorInsertHypoxia Responsive Elements (3)
UseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorAvailable SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags3XFLAG-Cas9ExpressionMammalianPromoterCBh; U6Available SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
KillerOrange /pQE-30
Plasmid#74748PurposeKillerOrange gene for bacterial expressionDepositorInsertKillerOrange
TagsHisTagExpressionBacterialMutationKillerRed with substitutions G3C Y66W D113S N145S…PromoterT5Available SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
Tags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianPromoterCAGAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange
Plasmid#177827PurposeHost Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)DepositorInsertmOrange
ExpressionMammalianPromoterCMVAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
OCT4-2A-mOrange
Plasmid#66986PurposeGenerate OCT4 mOrange reporter line without selection cassetteDepositorInsert2A-mOrange
Available SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …Available SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH2B-PSmOrange2
Plasmid#34960DepositorInsertPSmOrange2
TagsH2BExpressionMammalianMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only