164,577 results

1. pFRT_TO_FlagHA_G3BP1

   Plasmid

   #127106

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin and U6

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRosa26-EF1a-hCas9IRESneo

   Plasmid

   #67987

   Purpose

   Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-hCas9IRESneopA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-P11

   Plasmid

   #120022

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet-i
   

   Use

   Pcr template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-Cadherin-EGFP

   Plasmid

   #18870

   Depositor

   Valeri Vasioukhin

   Article

   Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

   Insert

   N-Cadherin (Cdh2 Mouse)
   

   Use

   Tags

   EGFP, Kanamycin, and N-Cadherin

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHM6-alphasynuclein-A53T

   Plasmid

   #40825

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-GRAB_ATP1.0

   Plasmid

   #167577

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-puro

   Plasmid

   #17923

   Purpose

   Insect expression vector

   Depositor

   David Sabatini

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Drosophila metallothionein gene promoter drives e…

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-TEAD1

    Plasmid

    #229411

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    TEAD1 (TEAD1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA6.2 EmGFP hAQP4(M23)

    Plasmid

    #126464

    Purpose

    Expresses human AQP4 (isoform M23) as an EmGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

    Insert

    Aquaporin 4 (AQP4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Promoter

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. EB3N-VVDfast-mCl3-iLID

    Plasmid

    #190165

    Purpose

    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    EB3N-VVDfast-mClover3-iLID
    

    Use

    Tags

    -VVDfast-mClover3-iLID

    Expression

    Mammalian

    Mutation

    1-189 aa from EB3

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. IronFistMUT.

    Plasmid

    #243008

    Purpose

    Iron binding deficient IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    H15A, H57A, E58A, E61A, H126A, E130A

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Iron Insensitive Control Construct 2

    Plasmid

    #243007

    Purpose

    Iron Insensitive Control Construct under the same expression promoter used in IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    mNeonGreen
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. IronFist

    Plasmid

    #243006

    Purpose

    Genetically encoded fluorescent iron reporter

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    Hemerythrin-like (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBiFCt-2in1-CC

    Plasmid

    #105114

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Use

    Tags

    cYFP-Myc/nYFP-HA

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9

    Plasmid

    #42876

    Purpose

    Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    tracr/Cas9
    

    Use

    CRISPR; E.coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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