159,141 results

1. pDONR223_TRAF3_WT

   Plasmid

   #81755

   Purpose

   Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TRAF3 (TRAF3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEH303 NLS dTomato 6x high affinity MS2 loops

   Plasmid

   #168215

   Purpose

   Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   NLS dTomato
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMD2-VSVGmut

   Plasmid

   #182229

   Purpose

   Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   VSVGmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP3664

   Plasmid

   #222350

   Purpose

   Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Gdf6

   Plasmid

   #163598

   Purpose

   Expresses GDF-6 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Gdf6 (Gdf6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL703

   Plasmid

   #140373

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-2BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSDMA25

   Plasmid

   #67940

   Purpose

   Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

   Type

   Empty backbone

   Use

   Cryptococcal complementation vector

   Tags

   Expression

   Mutation

   Promoter

   ACT1 for Cryptococcal marker

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES2-hPAC

   Plasmid

   #129488

   Purpose

   Expresses human TMEM206

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   Transmembrane protein 206 (PACC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLlacO-1-mfLon

   Plasmid

   #45152

   Depositor

   Michel Maharbiz

   Article

   Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

   Insert

   Lon
   

   Use

   Synthetic Biology

   Tags

   ssRA degradation (LVA)

   Expression

   Bacterial

   Mutation

   Promoter

   PLlacO-1

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid

    #207773

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMApra-Ptet1

    Plasmid

    #218360

    Purpose

    Empty backbone with tetR-PtetA expression system with single tetR operator site

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti-FLAG-CAMK2D

    Plasmid

    #221699

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-NLS-HA-Bxb1

    Plasmid

    #51271

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid

    #218193

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

    Depositor

    Florence Pojer

    Insert

    PNGaseF
    

    Use

    Tags

    tufA-His-CBD-3C

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-354

    Promoter

    T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUNIV backbone

    Plasmid

    #24705

    Depositor

    Cynthia Czajkowski

    Article

    Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

    Type

    Empty backbone

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDT-SuperFi-Cas9

    Plasmid

    #183630

    Purpose

    Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

    Depositor

    David Taylor

    Article

    Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

    Promoter

    T7

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJBL723

    Plasmid

    #140393

    Purpose

    Expresses HucR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-HucR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mito-V5-APEX2

    Plasmid

    #72480

    Purpose

    Expresses APEX2 in mitochondria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAct-PE2-HygroR

    Plasmid

    #149552

    Purpose

    Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Act5c

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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