Skip to main content
Addgene

We narrowed to 16,936 results for: She

Showing: 4451 - 4500 of 16936 results
  1. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-B*40:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Ef1alpha
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pD451-pA-APEX2

    Plasmid
    #157863
    Purpose
    Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling
    Depositor
    Insert
    pA-APEX2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pTCF7 HLA-B*07:02

    Plasmid
    #180446
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-B*07:02
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pBABE.is4-mOpn

    Plasmid
    #74410
    Purpose
    Expresses mouse Osteopontin from transcript tv4 in mammalian cells
    Insert
    mouse Opn (Spp1 Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pET32a-Trx-His6-TEV_SacB

    Plasmid
    #164588
    Purpose
    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.
    Depositor
    Insert
    TEV-SacB
    Use
    Tags
    Trx-His6 followed by TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pCIG3 Rab5CA

    Plasmid
    #78268
    Purpose
    Rab5 Constitutively active (Q79L) expression
    Depositor
    Insert
    RAB5A (RAB5A Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Q79L
    Promoter
    CMV, Lentiviral LTR
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. RABEP1A-c019

    Plasmid
    #175477
    Purpose
    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.
    Depositor
    Insert
    RABEP1 (RABEP1 Human)
    Use
    Tags
    TEV-TwinStrep
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  17. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  18. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pLIX403-EYFP-ccdB-Zeo

    Plasmid
    #158565
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pTCF5 HLA-A*01:01

    Plasmid
    #180448
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-A*01:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-B*35:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. pT7-V5-SBP-C1-HsPTB1

    Plasmid
    #64925
    Purpose
    Expresses V5- and SBP-tagged PTB1 in mammalian cells
    Depositor
    Insert
    PTB1 (PTBP1 Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  23. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    Insert
    Gene Expression Reporter Construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
  24. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
  25. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. pmr101A-cPr-mCherry (KmR)

    Plasmid
    #177207
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  27. Ptch1-mCherry

    Plasmid
    #118982
    Purpose
    Expresses mCherry tagged Ptch1 in mammalian cells
    Depositor
    Insert
    Ptch1 (Ptch1 Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    see depositor comments below
    Promoter
    CMV
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
  28. RAB5AA-c003

    Plasmid
    #175475
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-N215.
    Depositor
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pTCF6 HLA-A*24:02

    Plasmid
    #180445
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-A*24:02
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pFastBacMam3_N_His8_eGFP_3C

    Plasmid
    #178807
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His8, eGFP, 3C
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. AAV-GFAP-mNeonGreen

    Plasmid
    #105713
    Purpose
    AAV packaging vector to express mNeonGreen in astrocytes.
    Depositor
    Insert
    mNeonGreen
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Minimal GFAP promoter
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  32. pTCF48 HLA-A*11:01

    Plasmid
    #180449
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    HLA-A*11:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. BC-GPR56

    Plasmid
    #44197
    Depositor
    Insert
    human GPR56 (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    LTR
    Available since
    May 3, 2013
    Availability
    Academic Institutions and Nonprofits only
  34. pLVX-EGFP-C3-cenexin

    Plasmid
    #73334
    Purpose
    Lentiviral vector encoding cenexin with an N-terminal EGFP tag
    Depositor
    Insert
    cenexin/ODF2 (ODF2 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
  35. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  36. pLIX403-EBFP2-ccdB-NatMx

    Plasmid
    #158559
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  37. pQ2aB

    Plasmid
    #124887
    Purpose
    Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Blue Flourescent Protein, Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
  38. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid
    #178808
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Flag, His8, eGFP, TEV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. USH1CA1-TwinStrepII

    Plasmid
    #83202
    Purpose
    Expression of tagged protein in mammalian cells
    Insert
    USH1C (Ush1c Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  40. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  41. KPC2-pNic-Bsa4

    Plasmid
    #231082
    Purpose
    expresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tag
    Depositor
    Insert
    carbapenemase-2 (kpc2 )
    Use
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
  42. ORFtag_LambdaN-SD_Frame3

    Plasmid
    #220986
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame3
    Depositor
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  43. ORFtag_LambdaN-SD_Frame1

    Plasmid
    #220984
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame1
    Depositor
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  44. ORFtag_LambdaN-SD_Frame2

    Plasmid
    #220985
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a LambdaN tag followed by a splice donor sequence in Frame2
    Depositor
    Insert
    lambdaN
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  45. pLV V5-Sun2

    Plasmid
    #175114
    Purpose
    Lentiviral expression of V5-tagged mouse Sun2
    Depositor
    Insert
    Sun2 (Sun2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  46. HA-V2R

    Plasmid
    #37061
    Depositor
    Insert
    vasopressin 2 receptor (AVPR2 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
  47. pKSB- 3xP3-YFPsv40 GGGG AAGA

    Plasmid
    #174530
    Purpose
    3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Insert
    3xP3-YFP BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  48. pLenti- V6.3 Ultra

    Plasmid
    #106172
    Purpose
    lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable
    Depositor
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 4451 - 4500 of 16936 results