19,180 results

1. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-CAGGS-EGFP

   Plasmid

   #22212

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   CAGGS-eGFP
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FUW-tetO-NFIA

   Plasmid

   #141403

   Purpose

   Inducible Expression of NFIA

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCpf1b

   Plasmid

   #122187

   Purpose

   Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. human p53-(1-320)

   Plasmid

   #24864

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1-320

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pLKO.3G

   Plasmid

   #14748

   Depositor

   Diane Mathis, Christophe Benoist

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREP-NFAT2

   Plasmid

   #11788

   Depositor

   Anjana Rao

   Insert

   NFAT2 (NFATC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXS-IRES-Blast POLE1

   Plasmid

   #160802

   Purpose

   Express POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLE1 (POLE Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   shPOLE_1 and shPOLE2 resistant

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pIRES2-EGFP-SHP2WT

    Plasmid

    #12283

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2WT (PTPN11 Human)
    

    Use

    Tags

    Myc (not canonical: KLISEEDL)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-cMYC promoter

    Plasmid

    #114724

    Purpose

    reporter luciferase construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Oncogene. 1994 Feb;9(2):405-15.

    Insert

    C-Myc promoter region
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETDuet S2

    Plasmid

    #171921

    Purpose

    Express RNA-based (p)ppGpp sensor and a NirFP

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Inserts

    RNA-based (p)ppGpp sensor
    NirFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SIVu

    Plasmid

    #81089

    Purpose

    Single Integration Vector URA - Cloning vector for single integration into the URA3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-Brainbow-2.1 R

    Plasmid

    #18723

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. myc-Raptor

    Plasmid

    #1859

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-CRY2-5ptaseOCRL

    Plasmid

    #66836

    Purpose

    Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    5ptaseOCRL (OCRL Human)
    

    Use

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Shigeo Sugano, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-mNFM

    Plasmid

    #32909

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

    Insert

    EGFP-mouse NFM fusion (Nefm Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAM1

    Plasmid

    #51401

    Purpose

    Expresses truncated version of NLL-MetRS from endogenous MetG promoter

    Depositor

    David Tirrell

    Article

    Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Insert

    MetRS (metG E coli)
    

    Use

    Tags

    Expression

    Mutation

    L13N, Y260L, H301L; Please note that a truncated …

    Promoter

    MetG

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGP-CMV-NES-jRCaMP1a

    Plasmid

    #61562

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid

    #80768

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-DIO-EGFP

    Plasmid

    #50457

    Purpose

    Double floxed EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG_GPHN.FingR-eGFP-CCR5TC

    Plasmid

    #46296

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-eGFP-CCR5TC (GPHN Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge