We narrowed to 5,564 results for: SPI

1. pcDNA3-MTS-KD-c-Src-FLAG

   Plasmid

   #44653

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed lysine 298 to methionine

   Promoter

   CMV promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SBC009876

   Plasmid

   #226283

   Purpose

   Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

   Depositor

   Nigel Scrutton

   Article

   Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGPN-ACE2

   Plasmid

   #187483

   Purpose

   Expression vector - ACE2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ230-RVR

   Plasmid

   #108861

   Purpose

   LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G548R, K554V and Y558R, Cpf1 is rice codon optimi…

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTriEX-Antenna-GDI Rac1

   Plasmid

   #83359

   Purpose

   Expression of Antenna-Rac1

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   FRET Antenna (mCerulean-cpVenus) and His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINO_H11

   Plasmid

   #184277

   Purpose

   Expresses nanobody H11 in E. coli

   Depositor

   Ray Owens

   Article

   Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Nanobody H11
   

   Use

   Tags

   His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX304-MARK3_T211A

   Plasmid

   #107236

   Purpose

   Lentiviral gene expression vector for phospho-MARK3_T221A expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2-CMV/TetO2-EYFP-GW

   Plasmid

   #153309

   Purpose

   Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Yellow Fluorescent Protein

   Expression

   Mutation

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLPS1-iLID::EGFP::FTH1 (pBS1042)

   Plasmid

   #185289

   Purpose

   For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   iLID::eGFP::FTH1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid

    #114038

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    MAD2 binding deficient MAD1 K541A/L543A

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE-80L 2xHis-LRRK2 Armadillo 1-552

    Plasmid

    #186017

    Purpose

    2xHis-LRRK2 Armadillo 1-552

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-eDHFR-Cdc42Q61L

    Plasmid

    #107267

    Purpose

    mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-mScarlet-SARS-CoV-2-SL1 alone

    Plasmid

    #191485

    Purpose

    Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3019_pETcon-SARS-CoV-2-RBD_E484K

    Plasmid

    #184404

    Purpose

    yeast surface display of the SARS-CoV-2 Eta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    E484K

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ApoE3_mCh-SspB (pBS1144)

    Plasmid

    #185326

    Purpose

    For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SARS-CoV-2 Δ18 P.2/B.1.1.248

    Plasmid

    #173477

    Purpose

    Encodes SARS-CoV-2 P.2/B.1.1.248 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 Spike Truncated to remove 18 amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-7 asyn S87E

    Plasmid

    #36059

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-7 asyn S87A

    Plasmid

    #36058

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87A (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87A

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-C1 hSlp2-a del.SHD

    Plasmid

    #40050

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a del.SHD (SYTL2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of SHD domain (1-56)

    Promoter

    CMV promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pEGFP-C1 hSlp4-a del.SHD

    Plasmid

    #40036

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a del.SHD (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted SHD domain (1-143)

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

23. ApoE2_mCh-SspB (pBS1142)

    Plasmid

    #185324

    Purpose

    For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

    Plasmid

    #184406

    Purpose

    yeast surface display of the SARS-CoV-2 Beta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    K417N+E484K+N501Y

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX019

    Plasmid

    #167145

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal hispidin-3-hydroxylase, nnH3H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1 hSlp4-a C2AB

    Plasmid

    #40037

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2AB (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A and C2B domains (353-671)

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C1 hSlp4-a C2A

    Plasmid

    #40038

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2A (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A domain (353-487)

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1 hSlp4-a C2B

    Plasmid

    #40039

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2B (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2B domain (488-671)

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX4T-1 hSlp2-a del.C2AB

    Plasmid

    #40059

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a del.C2AB (SYTL2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of C2A and C2B domains (601-910)

    Promoter

    Tac

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pEGFP-C1 hSlp2-a V18A

    Plasmid

    #40054

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a V18A (SYTL2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Valine 18 to Alanine

    Promoter

    CMV promoter

    Available since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pGEX4T-1 Slp2-a C2AB

    Plasmid

    #40058

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a C2AB (Sytl2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C2AB domains only

    Promoter

    Tac

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pEGFP-C1 Slp2-a C2B

    Plasmid

    #40053

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a C2B (Sytl2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2B domain

    Promoter

    CMV promoter

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pET15b-ITPA_P32T

    Plasmid

    #60825

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P32T

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

34. CRY-GalVP16l (B694)

    Plasmid

    #92030

    Purpose

    expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    GalBD-VP16

    Expression

    Mammalian

    Mutation

    NLS sequence of CRY2 mutated

    Promoter

    CMV

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX4T-1 Slp2-a E11A/R32A

    Plasmid

    #40063

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a E11A/R32A (Sytl2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Glutamate 11 to Alanine, Arginine 32 to Alanine

    Promoter

    Tac

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pEGFP-C1 Slp2-a C2A

    Plasmid

    #40052

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp2-a C2A (Sytl2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A domains

    Promoter

    CMV promoter

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pEGFP-C1 hSlp4-a K>Q

    Plasmid

    #40044

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a K>Q (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Lysine 410,412,416,564,566,571, 572 to Glutamine

    Promoter

    CMV promoter

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pEGFP-C1 hSlp4-5-4a

    Plasmid

    #40045

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-5-4a (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Slp4 linker substituted by Slp5 linker domain (s…

    Promoter

    CMV promoter

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pEGFP-C1 hSlp4-a W118S

    Plasmid

    #40042

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a W118S (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Tryptophane 118 to Serine

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pEGFP-C1 hSlp4-a V21A

    Plasmid

    #40041

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a V21A (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Valine 21 to Alanine

    Promoter

    CMV promoter

    Available since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

41. pEGFP-C1 hSlp4-a SHD

    Plasmid

    #40034

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a SHD (SYTL4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    SHD domain (1-143)

    Promoter

    CMV promoter

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

42. TOPO-LG4_chr5:550kb antisense

    Plasmid

    #205468

    Purpose

    Enhancer containing plasmid used for assessing enhancer::promoter interactions

    Depositor

    Glen Borchert

    Article

    DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    chr5:550kb LG4
    

    Use

    Synthetic Biology; Subcloning of taq-amplified pc…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. BtARR1 Cys-less

    Plasmid

    #236274

    Purpose

    Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

    Depositor

    Vsevolod Gurevich

    Article

    Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

    Insert

    arrestin-1 (SAG Bovine)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C63A, C128S, C143A, inserted GGT codon after star…

    Promoter

    lac

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. V2R untagged

    Plasmid

    #208911

    Purpose

    encodes the human vasopressin receptor 2, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    AVPR2 (AVPR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. V1bR untagged

    Plasmid

    #208925

    Purpose

    encodes the human vasopressin receptor 1b, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    AVPR1B (AVPR1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENTR1A 2xHA GPR137

    Plasmid

    #226809

    Purpose

    Gateway entry vector for GPR137

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_001164351

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid

    #222876

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FRB chain: V84R in GPA

    Promoter

    MSCV LTR

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

    Plasmid

    #208170

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
    

    Use

    Tags

    His-tag, SUMO-tag, and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pOPINOH11-A10

    Plasmid

    #184278

    Purpose

    Expresses nanobody H11-A10 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-A10
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pOPINO_H11-B5

    Plasmid

    #184279

    Purpose

    Expresses nanobody H11-B5 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-B5
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge