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Plasmid#170726PurposeHR donor vector for ACTB gene with the revised marker sequence for ASHRA.DepositorInsertDonor sequence to ACTB gene for HR with the revised marker sequence
UseHr donor vector for human actb gene.TagsExpressionMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMEF2D-3XFlag
Plasmid#32965PurposeFor mammalian expression of mouse MEF2D with a 3x FLAG tag on the C-terminus.DepositorInsertMEF2D (Mef2d Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
YSCD44A-c002
Plasmid#175493PurposeProtein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.DepositorInsertSingle chain Fv CD44 antibody (H90)
UseTags3XFLAG-EK-His8 and pelBExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-DENV_NS3-GS-NS2B
Plasmid#201940PurposeExpresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertDENV NS3-GS-NS2B (POLY )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-WNV_NS2B-GS-NS3
Plasmid#203475PurposeExpresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorInsertWNV NS2B-GS-NS3 (POLY )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-CT10HF-LIC
Plasmid#39191PurposeSGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirusTagsTEV-His-FLAGExpressionMutationPromoterpolyhedrinAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
rnALDH
Plasmid#67920PurposeExpresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coliDepositorInsertRattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-K-red-SPOTIT
Plasmid#191490PurposeRed fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoterDepositorInsertK-red-SPOTIT
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-M-red-SPOTIT2
Plasmid#191491PurposeRed fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoterDepositorInsertM-red-SPOTIT2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLENTI_TetOn_Hu_ECT2(WT)
Plasmid#136339PurposeLentiviral inducible expression (TetOn) of human ECT2DepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pDsred monomer hygro C1- MLPH
Plasmid#89231PurposeExpression of tagged wild type human Melanophilin with Dsred monomer in mammalian cellsDepositorInsertHuman Melanophilin (MLPH Human)
UseTagsDsred monomerExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PVALB mAb_BCN14.1.1C9_HC_Rat IgG2a
Plasmid#182890PurposeMammalian expression of PVALB rat mAb HC geneDepositorInsertanti-PVALB (human) heavy chain (PVALB Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Lmx1a
Plasmid#45070DepositorInsertLmx1a LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pRNATU6.1 HDAC3 shRNA3
Plasmid#112272PurposeHDAC3 KDDepositorInsertshRNA against mouse HDAC3 (Hdac3 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-S1pr1-R120A
Plasmid#203707PurposeOverexpression of FLAG-S1PR1-R120A mutant.DepositorInsertFlag-S1PR1-R120A (S1PR1 Human)
UseTagsFlagExpressionMammalianMutationR120APromoterAvailable sinceDec. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]
Plasmid#100962PurposeContains genes encoding GPP synthase, cineole synthase and IspA(S81F)DepositorInsertGPPS-CSstr-ispA*
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-IFT20
Plasmid#118033PurposeExpresses human IFT20 in mammalian cellsDepositorInserthuman IFT20 (IFT20 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-CENPA-N-22
Plasmid#62752PurposeLocalization: Nucleus, N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertCENPA (CENPA Human)
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0
Plasmid#126080PurposesCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientationDepositorInserthChR2(H134R)-eYFP
UseAAVTagseYFPExpressionMutationH134RPromoterEf1aAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Lentiviral sgRNA human CD19
Plasmid#155289PurposeLentiviral expression of sgRNA against human CD19DepositorInsertCD19 (CD19 Human)
UseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-dDNMT3a-C706S-Hygro
Plasmid#104412Purposetransient expression of dCas9-dDNMT3a fusion proteinDepositorInsertdDNMT3a
UseCRISPRTagsExpressionMammalianMutationC706SPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-VQR
Plasmid#120224PurposepCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015DepositorInsertSpCas9-VQR
UseSynthetic BiologyTagsssrAExpressionMutationMutations D1135V/R1335V/T1337RPromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX400 Campylobacter lari CF89-12 Cas9
Plasmid#68336PurposeCampylobacter lari CF89-12 Cas9DepositorInsertClCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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SPR
Plasmid#39161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertsepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (SPR Human)
UseTagsHis TevExpressionBacterialMutationcodon-optimizedPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEO-CaMKII-EGFP
Plasmid#177018PurposeFor expression of EGFP in a single-floxed, excisable open reading frame (cre-off), under control of the CaMKIIa promoterDepositorInsertEGFP
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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px330_SMAD exon 2 gRNA
Plasmid#68350Purposepx330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.DepositorInsertSMAD exon 2 gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 636
Plasmid#53015PurposeAmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsfimE
fimB
UseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pE-SUMO CRYBA3
Plasmid#80747PurposeExpression of human beta A3-crystallin in E. coliDepositorInsertCRYBA3 (CRYBA1 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only