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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund, Benjamin Rost

Publication

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-PTRE-tight-hM3Dq-mCherry

Plasmid

#66795

Purpose

Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

pTIGHT promoter-hM3Dq-mCherry

Use

AAV

Expression

Mammalian

Promoter

pTIGHT TRE

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Publication

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCAG_GPHN.FingR-mKate2-IL2RGTC

Plasmid

#46297

Depositor

Don Arnold

Publication

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Publication

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Publication

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pFA6a-link-yomRuby2-SpHis5

Plasmid

#44858

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Publication

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 LIC cloning vector (6A)

Plasmid

#30124

Depositor

Scott Gradia

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Publication

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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Flag-TRIM24

Plasmid

#28138

Depositor

Michelle Barton

Publication

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pT2/BH

Plasmid

#26556

Depositor

Perry Hackett

Insert

pT2/BH

Expression

Bacterial

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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Ai6

Plasmid

#22798

Depositor

Hongkui Zeng

Publication

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed ZsGreen

Use

Mouse Targeting

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pENTR/pSUPER+ (575-1)

Plasmid

#17338

Purpose

Gateway entry vector for shRNA expression under H1 promoter

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pRK5-HA GST RagB wt

Plasmid

#19301

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pHCMV-10A1

Plasmid

#15805

Depositor

Miguel Sena-Esteves

Publication

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

10A1 Murine leukemia virus envelope mRNA

Expression

Mammalian

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5 alpha6

Plasmid

#16036

Depositor

Filippo Giancotti

Publication

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

alpha6 (ITGA6 Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 hE47

Plasmid

#16059

Depositor

Robert Benezra

Publication

Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

Insert

E47 (TCF3 Human)

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Publication

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin A (CCNA2 Human)

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Publication

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTP1005

Plasmid

#104160

Purpose

Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YopH (residues 164-468)

Plasmid

#79749

Purpose

This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

YopH (residues 164-468)

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDEST-FRT/T0-GFP-BRCA1

Plasmid

#71116

Purpose

mammalian expression of GFP-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Publication

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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