159,332 results

1. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Tatsuya Sawasaki, Shogo Nakano, Sohei Ito, Akira Nozawa

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Use

   Tags

   AGIA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Mito-ExRai-AMPKAR

   Plasmid

   #192448

   Purpose

   ExRai-AMPKAR targeted to the mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Mito-ExRai-AMPKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Promoter

   Available since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. fluoPEER-HEK3_5bp-del

   Plasmid

   #185476

   Purpose

   Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

   Depositor

   Sabine Fuchs

   Article

   Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

   Insert

   HEK3 editing site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Popt

   Plasmid

   #231705

   Purpose

   Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV P gene (codon-optimized )
   

   Use

   Vsv expression

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-Lopt

   Plasmid

   #231706

   Purpose

   Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV L gene (codon-optimized )
   

   Use

   Vsv expression

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSL1521 (pSPIN, pSC101* backbone)

   Plasmid

   #160729

   Purpose

   Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 Flag Pu.1

   Plasmid

   #66974

   Purpose

   Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

    Plasmid

    #184463

    Purpose

    pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.

    Depositor

    Rhonda Kineman

    Article

    Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757.

    Insert

    N-Flag-mSTAT5bCA (Stat5b Mouse)
    

    Use

    AAV

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Point mutation at base 1963 (A to C), which chang…

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pICH47742::p35S-ePEmax3::tEURb7

    Plasmid

    #227692

    Purpose

    Golden Gate level 1, position 2, ePEmax3 expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPSU250

    Plasmid

    #173837

    Purpose

    Expresses 250 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OPRM1-Tango

    Plasmid

    #66464

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    OPRM1 (OPRM1 Human)
    

    Use

    Tags

    FLAG and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBFC0993

    Plasmid

    #231993

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flag p21 WT

    Plasmid

    #16240

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTW2154

    Plasmid

    #236578

    Purpose

    ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    ISYmu1 TnpB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCap-SG5-Hgy

    Plasmid

    #227580

    Purpose

    Capture plasmid with hygromycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid

    #169247

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209723

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG RABV-G

    Plasmid

    #36398

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    rabies virus glycoprotein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGs

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

27. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225242

    Purpose

    Mammalian expression of iPASnFR-L in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge