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mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
HOXA9-MSCV-full-GFP

Plasmid

#20977

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

Insert

HOXA9 (Hoxa9 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Full length cDNA with ~1.1kb 3'UTR with 2 pu…

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

Plasmid

#193348

Purpose

tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
SEP-Nanoluc-cyOFP1

Plasmid

#141082

Purpose

prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc-cyOFP1

Use

Lentiviral

Tags

Expression

Mutation

Deletion of amino acids DISGG from positions 673 …

Promoter

EF1α

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCB410 (His-Ub-Ate1-4 1A7B)

Plasmid

#104339

Purpose

Express His Tag Ate1-4 1A7B

Depositor

Alexander Varshavsky

Insert

ATE1 (Ate1 Mouse)

Use

Tags

6xHis-Ub

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCWXPGR-pTF-Wnt3a

Plasmid

#114287

Purpose

TET-inducible expression of Wnt3a

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Talin-N-22

Plasmid

#54267

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Talin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

aa 268-476

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Talin-H-18

Plasmid

#57422

Purpose

Localization: Talin Head Domain, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin H

Use

Tags

mEos2

Expression

Mammalian

Mutation

Talin Head Domain

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TetO-FUW-Mecom 2

Plasmid

#118591

Purpose

Expresses Mecom isoform 2 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Mecom (Mecom Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mWnt6

Plasmid

#42281

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt6 (Wnt6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pCngA2-ISH

Plasmid

#15855

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
Unc5c.a-Fc-His

Plasmid

#72180

Purpose

Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.a (Unc5c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Co-Rest/RCOR1 [K72/8R]

Plasmid

#140066

Purpose

Mammalian Expression Plasmid of anti-Co-Rest/RCOR1 (Human). Derived from hybridoma K72/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Co-Rest/RCOR1 (Human) recombinant mouse monoclonal antibody (RCOR1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458_miR-290-295KO-gRNA1

Plasmid

#172709

Purpose

Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458_miR-290-295KO-gRNA2

Plasmid

#172710

Purpose

Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

miR-290-295

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

Plasmid

#105040

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BK beta1

Plasmid

#113565

Purpose

mouse BK beta1 subunit in mammalian expression vector

Depositor

Robert Brenner

Article

Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

Insert

Kcnmb1 (Kcnmb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSVL-mo FceRI gamma

Plasmid

#8372

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI gamma cDNA (Fcer1g Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pCHNFI-C2

Plasmid

#31403

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IC isoform 2 (Ctf2 Mouse)

Use

Tags

NA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR11 mSTIM2 myc

Plasmid

#17734

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM2 (Stim2 Mouse)

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
Sema6d.1-Fc-His

Plasmid

#72168

Purpose

Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.1 (Sema6d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAF211

Plasmid

#52407

Purpose

Expresses Flag-tagged mouse Arf6

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf6 (Arf6 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Sport6 Baf60b

Plasmid

#24723

Depositor

Benoit Bruneau

Article

Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

Insert

Baf60b (Smarcd2 Mouse)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pET16-mHP1alpha

Plasmid

#181910

Purpose

Bacterial protein expression vector for HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Anti-TrpV1 [N221/12R]

Plasmid

#188224

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/12

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAav-MCS-PQS2-3xHA

Plasmid

#84917

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

LOX-PGK-NEO-LOX

Use

AAV

Tags

PQS2 3xHA

Expression

Mutation

Promoter

mPGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 7: 5’3’ATF4.GFP

Plasmid

#21854

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Vector derived viral termination and poly-adenyla…

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Fbx15

Plasmid

#13353

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

F-box only protein 15 (Fbxo15 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pLJM60-Ptb

Plasmid

#38245

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Ptb (Ptbp1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac K-Flag Ring1A

Plasmid

#1995

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Kozak before start codon

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Klf5

Plasmid

#128830

Purpose

dox-dependent expression of mouse Klf5

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Klf5 (Klf5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mouse TF cDNA

Plasmid

#15454

Depositor

Nigel Mackman

Article

Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

Insert

Tissue factor (F3 Mouse)

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
FusionRed-VASP-5

Plasmid

#56136

Purpose

Localization: Focal Adhesions, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

VASP

Use

Tags

FusionRed

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Lenti-sgKmt2d#2/Cre

Plasmid

#173598

Purpose

Expresses a Kmt2d-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kmt2d (Kmt2d Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40

Plasmid

#186969

Purpose

PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.

Depositor

Brian Liau

Article

Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

Insert

Dnmt3a2 (Dnmt3a Mouse, Synthetic)

Use

Piggybac

Tags

Flag

Expression

Mammalian

Mutation

Isoform 2 (residues 220–908)

Promoter

CAG

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Jam1-AP-His

Plasmid

#71952

Purpose

Expresses the extracellular region of the JAM-1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam1 (F11r Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd9-1D4

Plasmid

#42271

Depositor

Jeremy Nathans, Chris Garcia

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 9 (Fzd9 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

Plasmid

#91880

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Ezh2 (Ezh2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Steven Clifford, Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS-GATA3

Plasmid

#34836

Depositor

Ellen Rothenberg

Article

Hernandez-Hoyos et al Immunity. 2003 Jul;19(1):83-94.

Insert

GATA3 (Gata3 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pMX-Brn4

Plasmid

#47028

Purpose

Expresses mouse Brn4

Depositor

Hans Schöler

Article

Han et al Cell Stem Cell. 2012 Apr 6;10(4):465-72.

Insert

Brn4 (Pou3f4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The insert includes 40 bp of the TOPO vector in t…

Promoter

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCWXPG-UBI-Glis3M412

Plasmid

#114301

Purpose

Ubiquitous expression of a constitutively active form of the Glis3 transcription factor

Depositor

Patrick Salmon

Insert

Glis3 (Glis3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

N-terminal deletion up to Methionine 412

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Use

Tags

VSV

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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