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Showing: 51 - 100 of 616 results

  1. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...community. MCC members gain access to mentors, virtual and local professional development, membership to a local...research opportunities to their students and other members of the MCC community. They support educators interested... CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a new window...

  2. AAVED

    Type
    Collection
    ...for Targeted Neuronal Manipulation Meeting Date September 21, 2018 Contact Us Questions? Contact us at AAVED...scientific outcomes. Event Details Date: Friday September 21, 2018, 9 AM - 5 PM Location: Addgene, 75 Sidney...workshops. Tentative Meeting Agenda Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect...Connect with Fellow Speakers and Attendees Friday, September 21 9:00 AM Breakfast and Welcome Melina Fan 9:...

  3. p53 Pathway

    Type
    Collection
    ...DR5 Tumor necrosis factor receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell...protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein...nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced...G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or ...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time...ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture large populations...Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model Organism...information about when genes are active in developing embryos. zfRegeneration - A database for gene expression...

  5. Ras Pathway

    Type
    Collection
    ...Resources Background Ras is a small GTPase and is a member of the G protein (guanine nucleotide binding protein...Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed in...RASSF8 RASSF9 RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase... in brain RHO RHOA RHOB RHOC Ras homolog family member ROCK ROCK1 ROCK2 Rho associated coiled-coil containing...

  6. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains.... as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander et al., Nat Biotechnol. 2011 )...individually or as a set as part of the Joung Lab REAL Assembly TALEN Kit , which also includes the four TALEN...

  7. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...

  8. Cloning

    Type
    Guide
    ...exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type IIS Assembly (Golden Gate...on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal cloning, more commonly... commonly known as Gibson assembly ( protocol ), takes advantage of the properties of 3 common molecular...DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40 base pair homology at...Gibson cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen orientation...into. A drawback of this system is that Gibson assembly works best when combining DNA fragments over 200...custom overhangs that can then be efficiently assembled together. The advantage of type IIS systems are...

  9. Synthetic Biology - Overview

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates and higher...System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart...

  10. Distribution to Industry

    Type
    Collection
    ...Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using...predict high accuracy junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet...Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene constructs...

  11. Antibody Plasmid Collection

    Type
    Collection
    ...plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain DNAs into a...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

  12. Optogenetics Guide

    Type
    Guide
    ...attaching only one member of the Zdk/LOV2 pair to the target protein, and the other to the membrane where the ...trafficking signals to localize opsins to the cell membrane. Microbial opsins, such as those described below... at physiological pH and trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic...from Kir2.1 and ER export signal provide improved membrane targeting 589 Archaerhodopsins Arch Light-activated...experimental contexts, including protein activation, membrane localization, and transcriptional activation. ...reversibly sequestering targets into large, optically assembled protein architectures in living mammalian cells...light-inducible dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), while its natural...

  13. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCCTGGCACAGGGGCTctccaccatgctggccAACCTGTTCTCAATGA kinesin-family-member-13a TAL3106 & TAL3107 TGTTCTCTGTTTGAGCGAgtctccacacagcagaGCGACAGTAACAGCTTCA...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487 TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA...

  14. Antibody Guide

    Type
    Guide
    ...Transfer proteins from the gel to a membrane. Incubate the membrane with an unconjugated primary antibody...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray...tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...recognizes your primary antibody source species. Remember that recombinant antibodies will also have a source...

  15. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and higher level gene networks...

  16. Addgene's eBook Collection

    Type
    Guide
    ...Select Plasmids 101 September 2023 | Fourth Edition About Plasmids 101 September 2023 | Fourth ...Science Career Guide November 2018 | First Edition About Science Career Guide November 2018 | First...

  17. Antibody Production

    Type
    Collection
    ...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(...cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...

  18. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to...pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A ...

  19. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...on an agarose gel Watch the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial Transformation ... new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...

  20. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin...

  21. Neurodegeneration Research Collection

    Type
    Collection
    ...Neurodegeneration Research Collection Addgene has assembled the following to help you find information and...cases having at least one other affected family member. These familial cases, usually inherited in an ...

  22. AAV Production in HEK293 Cells

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store... pH to ~8.5 Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start...supernatant as follows: Filter through a 0.45 μm PES membrane. Add 40% PEG such that the final PEG concentration...

  23. COVID-19 Resources

    Type
    Collection
    ...site for furin proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease...

  24. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology... Zhang's Lab ( Science , February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology...

  25. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...commonly added to aid in protein purification. Just remember to remove the stop codon for C-terminal tags and...yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - ...

  26. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction ...utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 ...

  27. Bacterial Expression Systems

    Type
    Collection
    ...GFP expression. Zinc Finger Consortium: Modular Assembly Accessory Reagents Kit Various Plasmids Sequence...bacterial two-hybrid (B2H) screen of modularly assembled zinc fingers. Return to Top Do you have suggestions...

  28. Molecular Biology Reference

    Type
    Guide
    ...nucleotide is present at the end of each fragment and assembles that information into the DNA sequence. Sanger...reactions on different pieces of DNA to be able to assemble the whole puzzle. That’s where Next Generation...sequenced templates are then aligned to each other to assemble the entire sequence or puzzle. It is important...

  29. General Transfection

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into sterile tubes. Store...limited effect on cell growth. Last reviewed on: November 7, 2023...

  30. Protocol - Bacterial Transformation

    Type
    Protocol
    ... still get enough colonies for your next step. Remember that each of these shortcuts will reduce the efficiency...field is applied to the cell/DNA mixture to induce membrane permeability. To do this you will need to have...

  31. Using a Light Microscope Protocol

    Type
    Protocol
    ...started in the lab! Introduction Microscopes are emblematic of biological research and are found in many ...focus. Pro-Tip As you increase your magnification, remember that the objectives are larger. Adjust your focus...

  32. Pipetting Protocol

    Type
    Protocol
    ...pipette tip and using the pipette. Last Update: September 2022 Video Watch the video for tips on pipetting...preloaded into tip boxes. In either case, once assembled into their boxes, pipette tips can be sterilized...

  35. Synthetic Biology - Algal

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  36. Synthetic Biology - Bacterial

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  37. Synthetic Biology - Fungal

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  38. Synthetic Biology - Mammalian

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  39. Synthetic Biology - Plant

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  40. Synthetic Biology - Worm

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  41. Synthetic Biology - Yeast

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse...

  42. Synthetic Biology - Strains

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  45. Synthetic Biology - Metabolism

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...

  47. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright Addgene 2006, All Rights Reserved... . 1996 272:263-267. PubMed . Asymmetry in the assembly of the RNAi enzyme complex. . Schwarz DS et. al...

  49. MAPK Plasmids

    Type
    Collection
    ...research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community. If you...
Showing: 51 - 100 of 616 results