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We narrowed to 125 results for: Toxoplasma gondii

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Showing: 1 - 50 of 125 results

  2. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  4. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

    Enlarge

  5. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

    Enlarge

  6. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  7. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

    Enlarge

  9. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

    Enlarge

  10. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

    Enlarge

  11. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

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  12. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

    Enlarge

  13. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

    Enlarge

  15. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

    Enlarge

  16. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

    Enlarge

  17. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

    Enlarge

  22. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

    Enlarge

  27. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available Since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

    Enlarge

  31. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

    Enlarge

  32. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available Since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Timothy Springer
    Insert
    M2AP
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

    Enlarge

  39. pFloxed DHFR-TS*

    Plasmid
    #100605
    Purpose
    pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR
    Depositor
    David Sibley
    Insert
    Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Mutation
    Pyrimethamine resistance
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100605-plasmid-map-sequence-id-192861

    Enlarge

  40. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

    Enlarge

  41. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87259
    Purpose
    YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-mAID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and mAID
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87259-plasmid-map-sequence-id-176555

    Enlarge

  42. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

    Enlarge

  47. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Promoter
    alpha-tubulin
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

    Enlarge

Showing: 1 - 50 of 125 results