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We narrowed to 5,133 results for: puc

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Showing: 5001 - 5050 of 5133 results

  10. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  14. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121062-plasmid-map-sequence-id-235983

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  16. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  17. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  18. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid
    #129794
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G102H
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129794-plasmid-map-sequence-id-255134

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  19. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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  20. pTB106-Hyg

    Plasmid
    #236779
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236779-plasmid-map-sequence-id-494138

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  21. pTB107-Phleo

    Plasmid
    #236782
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236782-plasmid-map-sequence-id-494137

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  31. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  32. Bxb1Donor_G315T_ADRB2_LucReporter

    Plasmid
    #129793
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G315T of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G315T
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129793-plasmid-map-sequence-id-255170

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  33. Bxb1Donor_W99A_ADRB2_LucReporter

    Plasmid
    #129789
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant W99A of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    W99A
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129789-plasmid-map-sequence-id-255165

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  34. Bxb1Donor_N51L_ADRB2_LucReporter

    Plasmid
    #129790
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant N51L of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    N51L
    Promoter
    N/A
    Available Since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129790-plasmid-map-sequence-id-255172

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  35. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87423-plasmid-map-sequence-id-173982

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  36. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  37. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

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  40. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  43. AICSDP-9:DSP-mEGFP

    Plasmid
    #87424
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    DSP Homology Arms with linker-mEGFP (DSP Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87424-plasmid-map-sequence-id-173979

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  44. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

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  45. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available Since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61576-plasmid-map-sequence-id-209910

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  50. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87427-plasmid-map-sequence-id-173980

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Showing: 5001 - 5050 of 5133 results