166,461 results

1. pLX_314-HRI-V5

   Plasmid

   #202434

   Purpose

   Expression of HRI

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-RAP1A-WT-puro

   Plasmid

   #156166

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIBR004

   Plasmid

   #177667

   Purpose

   SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

   Depositor

   John van der Oost

   Article

   Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pE6n

   Plasmid

   #17460

   Purpose

   modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   EYFP

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. mATF4

   Plasmid

   #24874

   Depositor

   Fumihiko Urano

   Article

   Ghosh et al PLoS One. 2010 . 5(3):e9575.

   Insert

   mouse ATF4 (Atf4 Mouse)
   

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pHT-6xR.ads-PxylA-DI

   Plasmid

   #47386

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   ads
   dxs
   idi
   

   Use

   Tags

   6xR and His

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac and PxylA

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-UbE2G2

   Plasmid

   #15791

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. K-SPOTIT1.0

   Plasmid

   #171215

   Purpose

   Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

   Depositor

   Wenjing Wang

   Article

   Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

   Insert

   K-SPOTIT1.0
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GreenGate 2.0 Toolkit

   Plasmid Kit

   #1000000241

   Purpose

   The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.

   Depositor

   Alexis Maizel

   Available Since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pGMC00010 (aka pRC0495)

    Plasmid

    #195295

    Purpose

    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRGEB31

    Plasmid

    #51295

    Purpose

    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBP-P_tetR-PTet*

    Plasmid

    #204077

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    tetR repressor and PTet* tetracycline-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry

    Plasmid

    #165828

    Purpose

    mCherry red fluorescent protein CDS

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

    Plasmid

    #110554

    Purpose

    miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    araC-ParaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC-ParaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-GluA2-Fab-LC

    Plasmid

    #230063

    Purpose

    Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab light chain fragment
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. mScarlet-I-UtrCH

    Plasmid

    #98823

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    UtrCH
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-RapGAP

    Plasmid

    #118324

    Purpose

    Expression of RapGAP

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RapGAP
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209663

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, David Root, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. FL-BCAN-lentiviral-GFP

    Plasmid

    #29704

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    Brevican (BCAN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

22. Tol2-krt4-GFP

    Plasmid

    #97151

    Purpose

    label apical epithelial cells in zebrafish

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Insert

    AcGFP
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLifeAct-mTurquoise2

    Plasmid

    #36201

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti CMV Neo DEST (705-1)

    Plasmid

    #17392

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eGFP L138 Reporter

    Plasmid

    #119130

    Purpose

    Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    mCherry and eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    eGFP L138S

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDF RBP-FL P53

    Plasmid

    #174042

    Purpose

    Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

    Depositor

    Stewart Loh

    Article

    Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

    Insert

    P53 gene (TP53 Human)
    

    Use

    Tags

    Thermoanaerobacter Tencongenesis ribose binding p…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAG1160 mCherry-FIREtag

    Plasmid

    #205164

    Purpose

    Expresses mCherry -FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMH-MBP

    Plasmid

    #101768

    Purpose

    Gateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion protein

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-6P-SEW-YC3.6

    Plasmid

    #31379

    Depositor

    Fritjof Helmchen

    Article

    Minderer et al J Physiol. 2011 Nov 14.

    Insert

    Yellow Cameleon 3.60
    

    Use

    AAV

    Tags

    Venus and eCFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230058

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230059

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-Puro-CLDN18

    Plasmid

    #69466

    Purpose

    Expresses CLDN18

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    CLDN18 (CLDN18 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep

    #121675-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Tags

    None

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

39. pDH3-PA-GFP

    Plasmid

    #18819

    Depositor

    Wei-Lih Lee

    Article

    Vorvis et al Yeast. 2008 Sep;25(9):651-9.

    Insert

    Photoactivatable Green Fluorescent Protein
    

    Use

    Yeast tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGES201

    Plasmid

    #190197

    Purpose

    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge