We narrowed to 4,529 results for: GCA

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide3

   Plasmid

   #125414

   Purpose

   CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3

   Plasmid

   #125410

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VEGFA-TSS-guide3

   Plasmid

   #125442

   Purpose

   CRISPR-mediated repression of VEGFA. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide3

   Plasmid

   #125422

   Purpose

   CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4B-TSS-guide1

   Plasmid

   #125420

   Purpose

   CRISPR-mediated repression of C2CD4B. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide4

   Plasmid

   #125415

   Purpose

   CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR VPS13C-TSS-guide1

   Plasmid

   #125412

   Purpose

   CRISPR-mediated repression of VPS13C. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide2

   Plasmid

   #125409

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide1

   Plasmid

   #125408

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3

    Plasmid

    #118179

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR OPTN TSS-guide1

    Plasmid

    #118181

    Purpose

    CRISPR-mediated repression of OPTN. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide4

    Plasmid

    #118176

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide1

    Plasmid

    #118177

    Purpose

    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217341

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217342

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KJ740(DE3)

    Bacterial Strain

    #179486

    Purpose

    KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Bacterial Resistance

    Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

    Species

    Escherichia coli

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

17. Syn61Δ3(ev5)

    Bacterial Strain

    #174514

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Bacterial Resistance

    Streptomycin

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

18. C-5545 ∆cosδε

    Bacterial Strain

    #209018

    Purpose

    Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

    Depositor

    Alfonso Jaramillo

    Article

    Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

    Bacterial Resistance

    Hygromycin

    Species

    Escherichia coli

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

19. XA92+pRCPam

    Bacterial Strain

    #195855

    Purpose

    E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.

    Depositor

    Gregory Phillips

    Article

    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

    Bacterial Resistance

    Chloramphenicol

    Species

    Acropora millepora

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

20. HL2483

    Bacterial Strain

    #62819

    Purpose

    Lim Lab strain: MG1655 + lacIq intergrated at intS + Pcon::TetR inserted into the chromosome at galK + ΔglgCAP + ΔcsrB + ΔcsrC + ΔcsrD

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Bacterial Resistance

    None

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

21. eScaf

    Bacterial Strain

    #220921

    Purpose

    cssDNA production strain

    Depositor

    Shawn Douglas

    Article

    Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

22. TB205 △proC △trpR

    Bacterial Strain

    #229546

    Purpose

    Fluorescently labelled (mCherry) E. coli, auxotrophic for proline, tryptophan overproducer

    Depositor

    Yolanda Schaerli

    Article

    Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

    Bacterial Resistance

    None

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

23. DHL708

    Bacterial Strain

    #98417

    Purpose

    Bacterial Strain with Genotype: MC4100 ∆clpPX

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Bacterial Resistance

    Streptomycin

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

24. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

25. HL3796

    Bacterial Strain

    #52951

    Purpose

    HL716 + ΔcsrA + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCD

    Depositor

    Han Lim

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

26. HL4018

    Bacterial Strain

    #52952

    Purpose

    HL716 + ΔcsrB + ΔcsrC + ΔcsrD + ΔglgCAP + ΔpgaABCD

    Depositor

    Han Lim

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

27. HL3796

    Bacterial Strain

    #37980

    Depositor

    Han Lim

    Bacterial Resistance

    Chloramphenicol

    Available since

    July 5, 2012

    Availability

    Academic Institutions and Nonprofits only

28. UQ6106

    Bacterial Strain

    #37176

    Depositor

    Gary Roberts

    Bacterial Resistance

    Ampicillin

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

29. HL3721

    Bacterial Strain

    #52950

    Purpose

    HL716 + ΔcsrB + ΔcsrC + ΔglgCAP + ΔpgaABCD

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

30. HL4142

    Bacterial Strain

    #37981

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Bacterial Resistance

    Chloramphenicol

    Available since

    July 5, 2012

    Availability

    Academic Institutions and Nonprofits only

31. TB204 △hisD

    Bacterial Strain

    #229548

    Purpose

    Fluorescently labelled (sfGFP) E. coli, auxotrophic for histidine

    Depositor

    Yolanda Schaerli

    Article

    Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

    Bacterial Resistance

    None

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

32. TB204 △metB

    Bacterial Strain

    #229549

    Purpose

    Fluorescently labelled (sfGFP) E. coli, auxotrophic for methionine

    Depositor

    Yolanda Schaerli

    Article

    Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

    Bacterial Resistance

    None

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

33. E. coli BW25113 ΔtnaA ΔtrpR

    Bacterial Strain

    #205015

    Purpose

    Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

    Depositor

    Yajun Yan

    Article

    Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Aug. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

34. Akaby

    Bacterial Strain

    #184224

    Purpose

    Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.

    Depositor

    Kate Adamala

    Article

    Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi

    Bacterial Resistance

    Kanamycin

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

35. Z956

    Bacterial Strain

    #200838

    Purpose

    Host strain for plasmids pYG205 and pYG215 or derivatives of the latter

    Depositor

    Boris Görke

    Article

    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

    Bacterial Resistance

    None

    Species

    Escherichia coli str. k-12 substr. mg1655

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

36. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

37. UQ6139

    Bacterial Strain

    #37177

    Depositor

    Gary Roberts

    Bacterial Resistance

    Ampicillin and kanamycin

    Species

    Burkholderia xenovorans

    Available since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

38. bSLS.114

    Bacterial Strain

    #191530

    Purpose

    Contains a knockout gene in the E. coli strain BL21-AI

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

39. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Jean-Loup Faulon, Chase Beisel

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

40. BL21 Rosetta2 ΔrecBCD

    Bacterial Strain

    #176583

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only