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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dUBA

Plasmid

#74205

Purpose

Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed aa 877 to encode for premature stop codon

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pMXspuro-GFP-NBR1-dLIR

Plasmid

#74204

Purpose

Retroviral expression of GFP-NBR1 with aa 727-738 deletion

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Deleted amino acids 727-738

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pCI-neo-Myc-hApg12

Plasmid

#22951

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Tags

3xMyc

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.0-Magneto2.0-p2A-mCherry

Plasmid

#74308

Purpose

Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Tags

Flag and p2A-mCherry

Expression

Mammalian

Mutation

TRPV4: delta 760–871

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA#1

Plasmid

#64245

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR2-EYFP

Plasmid

#67877

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-ABL

Plasmid

#31284

Depositor

Nora Heisterkamp

Article

Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Insert

V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ

Plasmid

#64875

Purpose

lentiviral expression of human POLQ

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Hmga2 3'UTR wt luciferase (Luc-wt)

Plasmid

#14785

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-wt (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pN1/G3BP1-iRFP

Plasmid

#129339

Purpose

Stress granule marker

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

iRFP (from Dr. M. Matsuda)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTG

Plasmid

#27437

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

histone-GFP-2A-TVA receptor-2A-rabies glycoprotein

Use

AAV; Adeno-associated viral

Mutation

N/A

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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MSP680

Plasmid

#65772

Purpose

Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135E, R1335Q, and T1337R mutations in Cas9

Promoter

CMV & T7

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET-PE2-His (IK1822)

Plasmid

#170103

Purpose

T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

Depositor

Keith Joung

Article

Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis

Use

CRISPR

Tags

6xHis tag

Expression

Bacterial

Mutation

Bacteria codon-optimized nSpCas9 (H840A) & M-…

Promoter

T7

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

Plasmid

#72666

Purpose

Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV6 BDNF

Plasmid

#39857

Depositor

Yves-Alain Barde

Article

Hofer et al EMBO J. 1990 Aug;9(8):2459-64.

Insert

BDNF (Bdnf Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt

Plasmid

#39531

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35516

Purpose

AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

Arch3.0-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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