179,537 results
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Plasmid#177937PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
ExpressionMammalianAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)
Viral prep#107312-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherry (physically separate, not a fusion protein)Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPEgRNA
Plasmid#172716PurposeDelivery of guide RNA for prime editingDepositorInsertgRNA
ExpressionBacterialAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_CoV2_B.1.1.7
Plasmid#170451PurposeExpress SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.DepositorInsertSARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))
TagsNAExpressionMammalianMutationHY69-70del, Y144del, N501Y, A570D, D614G, P681H, …PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX
Plasmid#167582PurposeExpress the ATP sensor GRAB_ATP1.0 in mammalian cellsDepositorAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV PHP.eB)
Viral prep#26973-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2 (AAV9)
Viral prep#99130-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDLX5/6TagsmRuby2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP13
Plasmid#141266PurposeGateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTarget_CAST
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-V5-miniTurboID-NES
Plasmid#116905PurposeExpresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertminiTurboID
UseDrosophila expressionAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-mCherry
Plasmid#114470PurposemCherry ControlDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInsertmCherry
UseAAVPromoterEf1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cilantro 2
Plasmid#74450PurposeCan clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralMutationWTPromoterU6Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (AAV1)
Viral prep#68719-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (#68719). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor with bicistronic mRuby2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG-FLEXTagsmRuby2 (Cre-dependent)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-nCas9-PBE
Plasmid#98163PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-nCas9-UGI-NLS
UseRiceExpressionPlantPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6His-MBP-TEV-huLbCpf1
Plasmid#90096PurposeBacterial expression plasmid for protein purificationDepositorInserthuLbCpf1
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.2-ccdB-Nanoluc
Plasmid#87075PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
Plasmid#83306PurposeLentivirus vector to express guideRNA and dCas9 with puro resistant geneDepositorInsertsgRNA and dCas9 from pX330
UseLentiviralTagsFLAG and PA tagsExpressionMammalianPromoterU6 for sgRNA and CBh for dCas9Available sinceDec. 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.2 TDP-43 NLS1 YFP
Plasmid#84912PurposeMammalian expresion of TDP-43 NLS1 YFPDepositorAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-hisMBP-AsCpf1-EC
Plasmid#79007PurposeExpressing his-MBP tagged AsCpf1 (E.coli codon optimized)DepositorInsertAsCpf1
Tags6xHis-MBP-NLS and NLS-HAExpressionBacterialPromotertac promoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB405
Plasmid#74799PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MIG-Luciferase-IRES-GFP
Plasmid#75021PurposeEncodes the expression of Luciferase and GFPDepositorInsertLuciferase
UseLuciferase and RetroviralAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GRM5-Tango
Plasmid#66390PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-Ii_SBP-EGFP-Golgin84
Plasmid#65303Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUGW-pHRed
Plasmid#65742PurposeLentiviral vector for pHRed expression in mammalian cells using the hUbiC promoterDepositorInsertpHRed
UseLentiviralExpressionMammalianPromoterhUbiCAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTLR repair vector
Plasmid#64322PurposeVector for repair of the traffic light reporter (TLR), providing the venus coding regionDepositorInsertVenus
UseArtificial targeting vectorMutationsee publication for detailsPromoternoneAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-SA-2A-NEO-CAG-RTTA3
Plasmid#60431Purposeneomycin resistance; constitutive promoter driving reverse tet activatorDepositorPublicationInsertrtTA3
UseZfn donorPromoterCAGAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIN-TetR-mCherry
Plasmid#59417Purposeretroviral construct expressing the Tet repressor (TetR) tagged with mCherryDepositorInsertTet repressor protein (TetR)
UseRetroviralTagsmCherryAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRosetta
Plasmid#59700PurposeLentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.DepositorInsertsPuro
BlastR
EGFP
UseLentiviralTags2AExpressionMammalianAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mApple-TOMM20-N-10
Plasmid#54955PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592DepositorAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH229: FCK-QuasAr1-mO2
Plasmid#51629PurposeFluorescent reporter for membrane voltage with improved brightnessDepositorInsertQuasAr1-mOrange
UseLentiviralTagsmOrange2ExpressionMammalianPromoterCaMKIIaAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4 huZAP(L)
Plasmid#45907DepositorInsertZAP(L) isoform (ZC3HAV1 Human)
TagsHAExpressionMammalianMutationM202T, L388F, Q701E, T851I (reference NP_064504.2)Available sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4-TO-Hygromycin-sfGFP-MAP
Plasmid#44100DepositorTypeEmpty backboneTagssfGFP-MAPExpressionMammalianAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)
Plasmid#40999DepositorTypeEmpty backboneTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFPExpressionMammalianPromoterCMVAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-UTX-F-MT2
Plasmid#40619PurposeExpression of enzyme-dead UTX in mammalian cellsDepositorPublicationInsertUTX (KDM6A Human)
Tags6xMyc and FlagExpressionMammalianMutationchanged Histidine 1146 and Glutamic Acid 1148 to …PromoterCMVAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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MSCV-N EBNA1
Plasmid#37954DepositorInsertEBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQCXIH-Flag-YAP-S127A
Plasmid#33092DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationS127APromoterCMV/MSVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p6599 MSCV-IP N-HAonly FOSL1
Plasmid#34897DepositorAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLAG-Ets2
Plasmid#28128DepositorPublicationAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEYFP-C1-NuMA
Plasmid#28238PurposeExpresses human NuMA with N-terminal EYFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
TagsEYFPExpressionMammalianPromoterCMVAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN_TmiRc3
Plasmid#25748PurposeEntry vector for cloning miR30-based shRNA driven by TRE promoter.DepositorTypeEmpty backboneUseGateway CloningAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by