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pCDNA 3 5' cMyc SMC1 wt

Plasmid

#32363

Depositor

Michael Kastan

Article

Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

Insert

SMC1A (SMC1A Human)

Tags

c-Myc

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Daniel Carlson, Stephen Ekker

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Mutation

Truncate at N and C terminous

Promoter

T3

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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aP2-luciferase

Plasmid

#8858

Depositor

Bruce Spiegelman

Article

Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

Insert

aP2 enhancer / promoter (Fabp4 Mouse)

Use

Luciferase

Tags

luciferase reporter

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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1318 pcDNA3 flag FKHRL1

Plasmid

#10708

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHRL1 (FOXO3 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pHIV-H2BmRFP

Plasmid

#18982

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

Histone H2B monomeric red fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 24, 2008

Availability

Academic Institutions and Nonprofits only

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pC-GoldyTALEN

Plasmid

#38143

Purpose

destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

Depositor

Daniel Carlson

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Tags

AcV5 and FokI homodimer

Expression

Mammalian

Mutation

Truncate at N and C terminous

Promoter

miniCaggs

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Article

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Tags

CaHygB

Promoter

TEF2

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Cherry-LacRep

Plasmid

#18985

Depositor

Mirek Dundr

Article

Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

Insert

LacI

Tags

AviTag, SV40 NLS, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pUC18

Plasmid

#50004

Purpose

pUC cloning vector

Depositor

Joachim Messing

Article

Norrander et al Gene. 1983 Dec;26(1):101-6.

Type

Empty backbone

Expression

Bacterial

Promoter

lac

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6

Plasmid

#39296

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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mRFP-Ub

Plasmid

#11935

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin (RBX1 Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-mCherry-T2A-Puro

Plasmid

#72264

Purpose

Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

Depositor

Kazuhiro Oka

Insert

mCherry-T2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only
GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Promoter

AtRPS5A

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMIEG3-JunDN

Plasmid

#40350

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

Jun dominant negative (JUN Human)

Use

Retroviral

Tags

HA and IRES eGFP

Expression

Mammalian

Mutation

first 122 amino acids deleted

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pBPGAL80Uw-6

Plasmid

#26236

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Expression

Insect

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-mPIAS1

Plasmid

#15206

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

Insert

PIAS1 (Pias1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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PKC delta DN

Plasmid

#16389

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta isoform 2 (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K376R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9-VP64

Plasmid

#47107

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

Flag, HA, SV40 NLS, and VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pBPLexA::p65Uw

Plasmid

#26231

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

LexA::p65 (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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