298,545 results

1. pMP220

   Plasmid

   #185921

   Purpose

   Broad host range IncP promoter probe using lacZ

   Depositor

   Herman Spaink

   Article

   Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

   Type

   Empty backbone

   Use

   Incp

   Tags

   LacZ

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET empty polycistronic destination vector (2E)

   Plasmid

   #29775

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET empty polycistronic destination vector (2E)

   Plasmid

   #29775

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Use

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGE144 (MutaEco; aTC-induced)

   Plasmid

   #189824

   Purpose

   Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

   Depositor

   Seokhee Kim

   Article

   Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

   Insert

   EcoRNAPα-PmCDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR/tetA promoter

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGE144 (MutaEco; aTC-induced)

   Plasmid

   #189824

   Purpose

   Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

   Depositor

   Seokhee Kim

   Article

   Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

   Insert

   EcoRNAPα-PmCDA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR/tetA promoter

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. bdSUMO-ZF5.3-GFP-HiBit

   Plasmid

   #170992

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. bdSUMO-ZF5.3-GFP-HiBit

   Plasmid

   #170992

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-CMV-mouse Gfi1-GFP

   Plasmid

   #91892

   Purpose

   3rd generation lentiviral expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Unknown

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-CMV-mouse Gfi1-GFP

    Plasmid

    #91892

    Purpose

    3rd generation lentiviral expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-SNAP-KanMX6

    Plasmid

    #87024

    Purpose

    Tag S. pombe genes in C terminal with SNAP

    Depositor

    Julien Berro

    Article

    Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-SNAP-KanMX6

    Plasmid

    #87024

    Purpose

    Tag S. pombe genes in C terminal with SNAP

    Depositor

    Julien Berro

    Article

    Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FIRES-PBCS-E670

    Plasmid

    #228699

    Purpose

    Sensor cassette FIRE2, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE2 SENSOR
    

    Use

    Synthetic Biology and Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hpgk

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. FIREC-PBCS-MR3

    Plasmid

    #228700

    Purpose

    Control cassette FIRE2, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE2 CONTROL
    

    Use

    Synthetic Biology and Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85866

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-17-3p Sensor (MIR17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85866

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-17-3p Sensor (MIR17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-2xSTREP-CDK6

    Plasmid

    #172614

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-HisD-miRFP720-HO1

    Plasmid

    #197257

    Purpose

    Expresses the protein of miRFP720 and HO1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQC-FsCFP-mCherryFP

    Plasmid

    #154836

    Purpose

    Frame-shift fluorescent reporter for genomic DNA editing

    Depositor

    Fangliang Zhang

    Article

    Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

    Insert

    targeting sequence of VEGF
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQC-FsCFP-mCherryFP

    Plasmid

    #154836

    Purpose

    Frame-shift fluorescent reporter for genomic DNA editing

    Depositor

    Fangliang Zhang

    Article

    Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

    Insert

    targeting sequence of VEGF
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. shLuc pRRL

    Plasmid

    #19125

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. shLuc pRRL

    Plasmid

    #19125

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PD-1-miSFIT-Ce67

    Plasmid

    #124680

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. PD-1-miSFIT-Ce67

    Plasmid

    #124680

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-mNRDc-V5

    Plasmid

    #51297

    Purpose

    expression of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc (Nrd1 Mouse)
    

    Use

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1-mNRDc-V5

    Plasmid

    #51297

    Purpose

    expression of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc (Nrd1 Mouse)
    

    Use

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pPOTv7-hygro-3HaloTag

    Plasmid

    #179828

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    3x HaloTag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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