161,697 results

1. pcDNA3.1 ZoAl N-1F

   Plasmid

   #203763

   Purpose

   For expression of N-flag tagged ZoAl R2 protein in mammalian cells

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   R2 retroelement protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK231 (AAVS1 CMV-MCS-PURO)

   Plasmid

   #105924

   Purpose

   Donor for integration at the human AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1X-Nup88

   Plasmid

   #87338

   Purpose

   To express human Nup88 in mammalian cells

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV5)

   Viral Prep

   #125712-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Tags

   mScarlet

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

6. pActin-miRFP670nano3

   Plasmid

   #184665

   Purpose

   Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-human beta-actin
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-BRIPO

   Plasmid

   #215542

   Purpose

   BRIPO (Bioluminescent Red Indicator for Potassium)

   Depositor

   Huiwang Ai

   Article

   Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

   Insert

   BRIPO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

9. R777-E229 Hs.RASA2

   Plasmid

   #70513

   Purpose

   Gateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASA2 (RASA2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-pBAD

    Plasmid

    #54630

    Purpose

    Localization: Protein Expression Vector , Excitation: 587, Emission: 610

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_HDLBP

    Plasmid

    #154448

    Purpose

    For recombination of HDLBP

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    HDLBP (HDLBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-AID-BLM-UGI-T2A-mTagBFP2

    Plasmid

    #221222

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaster1

    Plasmid

    #58525

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAG and EF1a

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

    Plasmid

    #198071

    Purpose

    The AAV vector for iRFP713(V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(V256C)
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mutation

    V256C mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Foxd3-pIRES2 eGFP

    Plasmid

    #37269

    Depositor

    Patricia Labosky

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMuLE ENTR CMV Renilla Luciferase L5-L2

    Plasmid

    #62186

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Renilla luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMT3-M

    Plasmid

    #26589

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    n/a
    

    Use

    Shuttle vector for mycobacteria

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmirGLO-3'UTR mouse Il6

    Plasmid

    #207126

    Purpose

    Luciferase vector containing 3'UTR for mouse Il6

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Interleukin 6 (Il6) (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKL11-GCaMP6f bb100

    Plasmid

    #158983

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23100 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. Anti-NPY [L115/13R] (Recombinant Antibody)

    Antibody

    #193439-rAb

    Purpose

    Anti-NPY (Human) recombinant mouse monoclonal antibody

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Recommended Applications

    Immunocytochemistry

    Reactivity

    Human, Mouse, and Rat

    Source Species

    Mouse

    Isotype

    IgG2a

    Trial Size

    Not available to purchase

    Available since

    April 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

23. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid

    #105537

    Purpose

    AAV expression of Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. KA1775_pPBCAG-rtTAM2-IH

    Plasmid

    #124167

    Purpose

    piggyBac-based expression of rtTA-M2

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    rtTAM2-IRES-Hph
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBJ5 mCnA alpha

    Plasmid

    #17871

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    CnA alpha (Ppp3ca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

27. TCARZ-CMV-GFP-PAmin-GF3-R4A22

    Plasmid

    #203768

    Purpose

    For production of template RNA for use in PRINT, GFP under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. R777-E233 Hs.RASAL1

    Plasmid

    #70517

    Purpose

    Gateway ORF clone of human RASAL1 [NM_001193521.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASAL1 (RASAL1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    R331H

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGHE-PdNIP2-1

    Plasmid

    #108447

    Purpose

    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

    Depositor

    Richard Bélanger

    Insert

    PdNIP2-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge