Addgene

We narrowed to 9,789 results for: otos

Showing: 3811 - 3840 of 9789 results

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pYI277

Plasmid

#228285

Purpose

Constitutive EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS4

Plasmid

#228450

Purpose

LS4 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS4

Use

Tags

10xHis-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS12

Plasmid

#228451

Purpose

LS12 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS12

Use

Tags

10xHis-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-PspCas13b-NES-mCherry

Plasmid

#233608

Purpose

Expression of PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

PspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-hNeuroD1

Plasmid

#233184

Purpose

Retroviral expression of NeuroD1

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

NeuroD1 (NEUROD1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-mBrn2

Plasmid

#233151

Purpose

Retroviral expression of Brn2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-hMyt1l

Plasmid

#233153

Purpose

Retroviral expression of Myt1l

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

Myt1l (MYT1L Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pC016-gPsp-EGFP_start_codon

Plasmid

#233613

Purpose

Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

gRNA targeting EGFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pB-DEST (JDW 1205)

Plasmid

#229821

Purpose

A PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Neo-DEST (JDW 940)

Plasmid

#229825

Purpose

A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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empty_pK7fWG2_IVD_FP611

Plasmid

#228371

Purpose

Empty dual expression vector for determining subcellular localization for protein of interest

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Type

Empty backbone

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-phiC31o (JDW 453)

Plasmid

#229842

Purpose

A CMV driven Phi31 recombinase.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

phiC31 codon optimized, PhiC31o

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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SOPP3-HA-nes

Plasmid

#229071

Purpose

Expresses SOPP3 in the cytosol of mammalian cells

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express SOPP3 in the cytosol of mammalian cells

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV4.51-mut

Plasmid

#201060

Purpose

Expresses AAV2 Rep and AAV4.51 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.51 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

N586R, S587V, N588D, L589R

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.52-mut

Plasmid

#201061

Purpose

Expresses AAV2 Rep and AAV4.52 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.52 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

N586A, S587G, N588F, L589R

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.18-mut

Plasmid

#201062

Purpose

Expresses AAV2 Rep and AAV4.18 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.18 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

K492E, K503E, N585S

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.68-mut

Plasmid

#201064

Purpose

Expresses AAV2 Rep and AAV4.68 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.68 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

T591V, D593R, R594G, T596R

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.69-mut

Plasmid

#201065

Purpose

Expresses AAV2 Rep and AAV4.69 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.69 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

T591P, D593M, R594G, T596G

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Use

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgScLEU2

Plasmid

#224868

Purpose

Disruption of S. cerevisiae type LEU2 gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mTagBFP2 (JDW 871)

Plasmid

#224531

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mTagBFP2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKanB

Plasmid

#223523

Purpose

Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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A99-ELP

Plasmid

#170760

Purpose

A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

A99-ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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AG73-ELP

Plasmid

#170762

Purpose

AG73 laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

AG73-ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF1m-ELP

Plasmid

#170763

Purpose

EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

EF1m-ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSN667

Plasmid

#219963

Purpose

Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

Depositor

Shinsuke Niwa

Insert

codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)

Use

Tags

sfGFP::2xStrepII tag

Expression

Insect

Mutation

encodes 1-721

Promoter

polyhedrin

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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