167,825 results

1. pXint129

   Plasmid

   #178208

   Purpose

   Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

   Depositor

   Gregory Phillips

   Article

   Platt et al Plasmid. 2000 Jan . 43(1):12-23.

   Insert

   xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)
   

   Expression

   Bacterial

   Promoter

   lacI

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN1-COX8-mScarletI

   Plasmid

   #129341

   Purpose

   Mitochondria matrix localization

   Depositor

   Yasushi Okada

   Insert

   mScarlet(i) (Addgene 85044)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe zeo HMGA2

   Plasmid

   #17411

   Depositor

   Tyler Jacks

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   HMGA2 (Hmga2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-EFNB2 (WT)

   Plasmid

   #200975

   Purpose

   Mammalian expression plasmid for wild type, myc-tagged EFNB2

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EFNB2 (EFNB2 Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1_V5_APEX_FLAG_Ct

   Plasmid

   #194069

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG-APEX-V5

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1 Bach1 S990A

   Plasmid

   #73643

   Purpose

   Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

   Depositor

   Sharon Cantor

   Insert

   Bach1 (BACH1 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   S990A (BRCA binding mutant)

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pLB038 - PHS1∆P

   Plasmid

   #136285

   Purpose

   entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

   Depositor

   Alexis Maizel

   Article

   Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

   Insert

   PHS1∆P
   

   Expression

   Bacterial

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPOTv7-blast-3HA

   Plasmid

   #221048

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   3xHA epitopes
   

   Use

   Unspecified

   Promoter

   read through

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0943

   Plasmid

   #143246

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LMO2 (LMO2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF0185 Cp36_attP-mCherry donor

    Plasmid

    #193468

    Purpose

    Promoterless mcherry donor for Cp36

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. RXFP2-Tango

    Plasmid

    #66493

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP2 (RXFP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTORC1 Focused library

    Pooled Library

    #164084

    Purpose

    Lentiviral CRISPR library targets human mTORC1 signaling pathway

    Depositor

    David Sabatini

    Article

    Condon et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

14. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECE-M2-PP1Cbeta

    Plasmid

    #31677

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein phosphatase 1, catalytic subunit, beta isozyme (PPP1CB Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pBiT3.1-C_PALB2:1-1186

    Plasmid

    #211160

    Purpose

    Mammalian expression of full-length human PALB2. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PALB2:M1-S1186 (PALB2 Human)
    

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. RXFP4-Tango

    Plasmid

    #66495

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP4 (RXFP4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCS2 xenopus chordin myc

    Plasmid

    #24682

    Depositor

    Edward De Robertis

    Article

    Piccolo et al Cell. 1996 Aug 23. 86(4):589-98.

    Insert

    Chordin
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDONR221-SV2C_STOP

    Plasmid

    #161463

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SV2C (SV2C Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pGEX-4T-1 3xHA-MKK6-EE

    Plasmid

    #47579

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a-Hisx6-Sirt6

    Plasmid

    #20277

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pDONR221-SLC17A8_STOP

    Plasmid

    #161127

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC17A8 (SLC17A8 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. mEmerald-DZIP1-N-18

    Plasmid

    #54070

    Purpose

    Localization: Centrosomes, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    DZIP1 (DZIP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

26. pBM16

    Plasmid

    #45344

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Tags

    NHA

    Expression

    Mammalian

    Available Since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

28. ITGB2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176983

    Purpose

    Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    ITGB2 (ITGB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR223_CSNK1E_WT

    Plasmid

    #82299

    Purpose

    Gateway Donor vector containing CSNK1E, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CSNK1E (CSNK1E Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDR-AmTrac

    Plasmid

    #47770

    Purpose

    expresses AmTrac in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac
    

    Tags

    mcpGFP

    Expression

    Yeast

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWZL Neo Myr Flag BEGAIN

    Plasmid

    #20567

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BEGAIN (BEGAIN Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

32. CMV-Rps7-meGFP

    Plasmid

    #241318

    Purpose

    Mouse Rps7 fused to meGFP for expression in mammalian cells

    Depositor

    Stavros Lomvardas

    Insert

    Rps7 (Rps7 Mouse)
    

    Tags

    meGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid

    #246438

    Purpose

    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 16 to Alanine, deleted amin…

    Promoter

    EF-1 alpha and U6

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid

    #246436

    Purpose

    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    

    Tags

    GFP N-terminal sequence (M1 to Q95), Hemi Intron …

    Expression

    Mammalian

    Mutation

    GFP N-terminal sequence M1 to Glutamine 95 append…

    Promoter

    CMV and U1a

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid

    #246435

    Purpose

    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    

    Tags

    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 60 to Alanine, changed Hist…

    Promoter

    CMV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247263

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247261

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL4)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

    Plasmid

    #247251

    Purpose

    Measure ISCro4 activity via luciferase expression

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FEOX-PBCS-TBF2- MC2

    Plasmid

    #234406

    Purpose

    Tool for genetically encoded fluorescent biosensor of cellular iron microenvironment

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Sci Rep. 2025 Oct 21;15(1):36596. doi: 1

    Inserts

    FEOX1 CONTROL
    FEOX
    

    Use

    Synthetic Biology

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1-alpha and hpgk

    Available Since

    Oct. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLPC-Myc-BirA

    Plasmid

    #234039

    Purpose

    Expresses Myc-His-BirA(R118G)

    Depositor

    Roderick O'Sullivan

    Article

    Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

    Insert

    BirA
    

    Use

    Retroviral

    Tags

    Myc-6xHis

    Expression

    Mammalian

    Mutation

    R118G

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge