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pEGFP-Syx2

Plasmid

#35705

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRFP-Syx1

Plasmid

#35707

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Expression

Mammalian

Promoter

RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Expression

Mammalian

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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M16 pCS-twhh-bGFP

Plasmid

#17153

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

bGFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-2

Plasmid

#24414

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pZH512

Plasmid

#102666

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-mPlexinD1

Plasmid

#16424

Depositor

Jonathan Epstein

Article

Gitler et al Dev Cell. 2004 Jul . 7(1):107-16.

Insert

Plexin D1 (Plxnd1 Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pNB777

Plasmid

#60153

Purpose

Red click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBR

Expression

Yeast

Promoter

LEU1pr

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Rab18b cds in pGem-T Easy

Plasmid

#80539

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18b (rab18b Zebrafish)

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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Rab18a cds in pGem-T Easy

Plasmid

#80538

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18a (rab18a Zebrafish)

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCL20-pre-mGRASP

Plasmid

#173117

Purpose

Expresses CCL20 sender construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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