We narrowed to 10,954 results for: aga

1. RGS-6xHis-mRFP-pcDNA3.1-

   Plasmid

   #52516

   Purpose

   expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108849

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCK072 p5E 147bp exorh:EGFP

   Plasmid

   #195950

   Purpose

   Gateway p5E vector with reporter

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh minimal regulatory elements and EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCold I-Hero7

   Plasmid

   #187926

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero7

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero7 (SERF2 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DJ-1 TEV site pET15b

   Plasmid

   #60687

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   TEV site was inserted (see comments below).

   Promoter

   T7

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

   Plasmid

   #108848

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcFLAG-CSK-R107E

   Plasmid

   #74503

   Purpose

   Overexpression of CSK R107E mutant in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R107E, K222R

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pM-ErbB4CTF

    Plasmid

    #17797

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment only, amino acids 676-1…

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-synp-F-H2B-GCaMP6f

    Plasmid

    #102994

    Purpose

    Expresses histone fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Article

    Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

    Insert

    H2B-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tol2-LyzC-GFP-U6ac-cdk2-guides

    Plasmid

    #168250

    Purpose

    "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    cdk2 sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

    Plasmid

    #108846

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-SP110A

    Plasmid

    #65392

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110A
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108845

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GAI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tol2-LyzC-RacFRET-U6ac-rac2 guides

    Plasmid

    #168248

    Purpose

    "label active Rac under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    RacFRET
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108843

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GID1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Myc-Foxo1-WT 6KR

    Plasmid

    #17558

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KR (Foxo1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K242R, K246R, K259R, K262R, K271R, K291R

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

    Plasmid

    #127775

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox10 (SOX10 Chicken)
    

    Use

    Enhancer reporter construct

    Tags

    Avi

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZLCv2-gD4Z4-3-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106354

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

    Plasmid

    #107858

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 2) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAcGFP1-N1-RvCAHS8-GS

    Plasmid

    #192461

    Purpose

    Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sgFFL

    Plasmid

    #59877

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DsRed +9e

    Plasmid

    #191766

    Purpose

    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Use

    Tags

    N terminal fusion of positively charged polypetod…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myc-hRAD18 C207F

    Plasmid

    #68829

    Purpose

    Expresses c-myc-tagged human RAD18 with C207F mutation

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 with C207F mutation (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pSMP-MeCP2_1

    Plasmid

    #36377

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MeCP2 (MECP2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

28. PX458_DMAP1_2

    Plasmid

    #86355

    Purpose

    Encodes gRNA for 3' target of human DMAP1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against DMAP1 (DMAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PX458_DMAP1_1

    Plasmid

    #86354

    Purpose

    Encodes gRNA for 3' target of human DMAP1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against DMAP1 (DMAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106352

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge