Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

169,450 results

Showing: 561 - 600 of 169450 results

  2. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

    Enlarge

  6. ADRA1A-Tango

    Plasmid
    #66213
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA1A (ADRA1A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66213-plasmid-map-sequence-id-369270

    Enlarge

  15. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

  17. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid
    #231953
    Purpose
    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    tTA_IRES_tagBFP
    Use
    Lentiviral
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231953-plasmid-map-sequence-id-470256

    Enlarge

  22. pHAGE-CMV-Flag-AAK1_IDG-K

    Plasmid
    #135280
    Purpose
    Gateway destination clone of AAK1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-AAK1 (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135280-plasmid-map-sequence-id-311570

    Enlarge

  29. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid
    #49518
    Purpose
    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38S8-P750-luxAB-DAS+4
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Promoter
    P750
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49518-plasmid-map-sequence-id-73896

    Enlarge

  30. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

    Enlarge

  31. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118733-plasmid-map-sequence-id-229276

    Enlarge

Showing: 561 - 600 of 169450 results