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Showing: 751 - 800 of 11158 results

  5. MtPT4-p3

    Plasmid
    #160003
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160003-plasmid-map-sequence-id-323050

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  7. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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  11. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Jerry Stitzel, Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

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  13. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  18. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212830
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212830-plasmid-map-sequence-id-434915

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  19. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212831
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212831-plasmid-map-sequence-id-434913

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  20. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164555-plasmid-map-sequence-id-330129

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  25. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pL452-Sf3b1-K700E

    Plasmid
    #90425
    Purpose
    selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90425-plasmid-map-sequence-id-182684

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  29. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid
    #154196
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC1-A, sgRNA-BC1-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154196-plasmid-map-sequence-id-308676

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  30. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  33. NbPT5b-p3

    Plasmid
    #160001
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbPT5b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160001-plasmid-map-sequence-id-323057

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  34. NbBCP1b-p3

    Plasmid
    #160002
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the NbBCP1b promoter for visualisation of arbuscular mycorrhizal colonisation in Nicotiana benthamiana roots
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    Bar (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Agrobacterium tumefaciens Nopaline synthase Promo…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160002-plasmid-map-sequence-id-323033

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  41. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

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  43. pLKO_HA.NWS.VSVg_NGFR

    Plasmid
    #158243
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158243-plasmid-map-sequence-id-329479

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  44. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

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  45. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid
    #158233
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158233-plasmid-map-sequence-id-327650

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  47. pL452(hygro)-Sf3b1-K700K

    Plasmid
    #90426
    Purpose
    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90426-plasmid-map-sequence-id-190769

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  48. pLKO_S.NWS.VSVg_NGFR

    Plasmid
    #158348
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.NWS.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158348-plasmid-map-sequence-id-330894

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  49. pLKO_FLAG.Ollas.VSVg_NGFR

    Plasmid
    #158324
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.Ollas.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158324-plasmid-map-sequence-id-330232

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  50. pLKO_S.FLAG.VSVg_NGFR

    Plasmid
    #158345
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158345-plasmid-map-sequence-id-330661

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Showing: 751 - 800 of 11158 results