We narrowed to 11,252 results for: NSI

1. p270 pCMV-CRE-M-AR(LNCaP)

   Plasmid

   #12496

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Insert

   CRE-M AR (AR Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Thr 868 Ala in AR-LBD

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pGL-p21UTRm2

   Plasmid

   #20879

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR site 2 mutation (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Predicted miRNA binding site 2 (Position ~1100) G…

   Promoter

   Available since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

   Plasmid

   #86195

   Purpose

   U6 based expression of N meningitidis sgRNA

   Depositor

   Scot Wolfe

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   nmCas9 sgRNA cloning cassete
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_DOCK2-1/1

   Plasmid

   #91482

   Purpose

   Protein expression and purification of human SH3 domain construct DOCK2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   DOCK2-1/1 (DOCK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_AMPH-1/1

   Plasmid

   #91556

   Purpose

   Protein expression and purification of human SH3 domain construct AMPH-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   AMPH-1/1 (AMPH Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_DBNL-1/1

   Plasmid

   #91409

   Purpose

   Protein expression and purification of human SH3 domain construct DBNL-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   DBNL-1/1 (DBNL Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

   Plasmid

   #140227

   Purpose

   Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HRE (Hypoxia inducible)

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST27-GST-ATXN1 FL(2Q)

   Plasmid

   #21752

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains 2Q (2 glutamines) and there isno …

   Promoter

   CMV

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pHH0103_ARHGEF16-1/1

   Plasmid

   #91334

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGEF16-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGEF16-1/1 (ARHGEF16 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHH0103_ARHGEF26-1/1

    Plasmid

    #91342

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF26-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF26-1/1 (ARHGEF26 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMKO.1-puro/hFOXH1 shRNA1

    Plasmid

    #59303

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. DPYS-FLAG

    Plasmid

    #61618

    Purpose

    To study the effect of DPYS activity on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidinase (DPYS Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MAC_N_ARPC1B

    Plasmid

    #111681

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    ARPC1B (ARPC1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAav-MCS-PQS1-3xFLAG

    Plasmid

    #84883

    Purpose

    rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

    Insert

    Multicloning sites and selection cassette
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BRCA1-(498-663)

    Plasmid

    #35070

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-663

    Promoter

    T7

    Available since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

17. BRCA1-(1316-1538)

    Plasmid

    #35079

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1316-1538

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pBT259_(pRosa26-G-tTA2)

    Plasmid

    #36878

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    diphteria toxin A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3b(1454/3172)P2P-wt

    Plasmid

    #21404

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pVL44 - pVND7>>

    Plasmid

    #116010

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BRCA1-(540-758)

    Plasmid

    #35071

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 540-758

    Promoter

    T7

    Available since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pBp-FGFR2c-W290C

    Plasmid

    #45701

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    W290C

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

23. VP-hAR1-660

    Plasmid

    #89081

    Purpose

    mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids linked to VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Langley et al J Biol Chem. 1998 Jan 2;273(1):92-101.

    Insert

    hAR-1-660 (AR Human)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    aa 1-660 (contains exons A,B,C and part of D)

    Promoter

    SV40

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

24. EGFP-CCHD (p130Cas)

    Plasmid

    #145132

    Purpose

    Expresses CCH domain from p130Cas with an EGFP tag

    Depositor

    Johanna Ivaska

    Article

    Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

    Insert

    p130Cas CCH domain (BCAR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEJS664-pMCSG7-AcrIIC5Smu

    Plasmid

    #113441

    Purpose

    Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXs-hFOXH1GR

    Plasmid

    #59311

    Purpose

    Forced expression of human FOXH1GR

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Retroviral

    Tags

    cGR - hormone binding domain of glucocorticoid re…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_CRKL-2/2

    Plasmid

    #91364

    Purpose

    Protein expression and purification of human SH3 domain construct CRKL-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRKL-2/2 (CRKL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBp-FGFR2b-P253R

    Plasmid

    #45716

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P253R

    Promoter

    Available since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

    Plasmid

    #79914

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. C-His-GvpC-TEV

    Plasmid

    #153296

    Purpose

    Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    TEV-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) 21 bp TEV recognition and cleavage site flanke…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBp-FGFR2c-K660N

    Plasmid

    #45708

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660N

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153540

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BRCA1-(360-498)

    Plasmid

    #35068

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-360

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

35. p274 pCMV-CRE-M-AR(T)

    Plasmid

    #12497

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M AR (AR Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Early termination mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

36. pAM4663

    Plasmid

    #85126

    Purpose

    NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

    Depositor

    Susan Golden

    Article

    Chang et al Science. 2015 Jul 17;349(6245):324-8. do

    Insert

    PkaiB-luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPR v2-sgDnmt1_1

    Plasmid

    #138347

    Purpose

    Express guide RNA 1 for Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiCRISPR v2-sgDnmt1_2

    Plasmid

    #138348

    Purpose

    Express guide RNA 2 for mouse Dnmt1

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    mouse Dnmt1 sgRNA (Dnmt1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST40-2XHA-Src-S4-UD-S32

    Plasmid

    #140302

    Purpose

    Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the KD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST40-2XHA-Src-S4-S32-KD

    Plasmid

    #140298

    Purpose

    Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge