We narrowed to 25,959 results for: Vit;

1. pCheck2 Dest (R1-R2) #BGV129

   Plasmid

   #48955

   Purpose

   Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Luciferase; Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE-eGFP-OPTN S513A

   Plasmid

   #175764

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S513A

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCardinal-CytERM-N-17

   Plasmid

   #56158

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   CytERM
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pSADdeltaG-GFP-AlstR

   Plasmid

   #32647

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   AlstR (AstA-R1 Fly)
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

5. SaKKH-ABE8e-dimer

   Plasmid

   #138503

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LbABE8e-dimer

   Plasmid

   #138505

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-dLbCas12a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGs-Hoxc8

   Plasmid

   #45555

   Depositor

   Jeremy Dasen

   Article

   Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

   Insert

   Hoxc8 (HOXC8 Chicken)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV / b-actin

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pRSETa-Amrose v3

   Plasmid

   #79539

   Purpose

   pRSETa (Invitrogen) plasmid expressing Amrose variant 3

   Depositor

   Randolph Caldwell

   Article

   Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

   Insert

   Amrose v3
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLL7.0: Venus-oLID-Mito (From ActA)

   Plasmid

   #60414

   Purpose

   Mammalian Expression of Venus-oLID-Mito

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-oLID-Mito
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-mU6-Luc2GO-PGK-Neo

    Plasmid

    #136905

    Purpose

    Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    Luc2CyGo
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-miRE_shBRD9_508-SV40-GFP

    Plasmid

    #75138

    Purpose

    Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBRD9_508
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Split-RESCUE-N

    Plasmid

    #170153

    Purpose

    AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-RESCUE-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-HA-mIFITM1-C49,50,83,103A

    Plasmid

    #58421

    Purpose

    Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 49, 50, 83, and 103 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

    Plasmid

    #91873

    Purpose

    Photo-activatable/light sensitive Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCASP-SptP120-E3_5-HilA

    Plasmid

    #153326

    Purpose

    Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-E3_5 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR sgSLC19A1

    Plasmid

    #102314

    Purpose

    genetic depletion of SLC19A1

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgSLC19A1 (SLC19A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. IBMc097

    Plasmid

    #161945

    Purpose

    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-SLC30A10-E25A

    Plasmid

    #82345

    Purpose

    Expresses FLAG-tagged SLC30A10-E25A in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    E25A

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKDsgRNA-rpsL

    Plasmid

    #89953

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rpsL gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPK1E-1+t

    Plasmid

    #48147

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid

    #108696

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C-terminal truncation (7aa) removing PIP box

    Promoter

    tac

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX6P1‐Afu‐rnhB D101N

    Plasmid

    #108695

    Purpose

    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    rnhB
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D101N catalytic site mutation

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKDsgRNA-ldhA

    Plasmid

    #102288

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ldhA gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-T5-ASG_ClpXP

    Plasmid

    #153299

    Purpose

    Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    ASG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCASP-SptP120-K27-HilA

    Plasmid

    #153328

    Purpose

    Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K27 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-h-mmPtch1-B

    Plasmid

    #120888

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-3xFLAG-Puro PAK5 E144K

    Plasmid

    #167193

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 E144K

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 E144K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-3xFLAG-Puro PAK5 M173I

    Plasmid

    #167192

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 M173I

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 M173I
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SaABE8e-dimer

    Plasmid

    #138501

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEGFP-hSyntenin2b

    Plasmid

    #89437

    Purpose

    mammalian expression of EGFP tagged human Syntenin2b

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *see below

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. enAsABE8e-dimer

    Plasmid

    #138507

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. FLAG-SLC30A10-N43H

    Plasmid

    #82344

    Purpose

    Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N43H

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAGGS-PB1-MontiRed-LOV2dark

    Plasmid

    #178874

    Purpose

    Mammalian expression of PB1-MontiRed-LOV2dark

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-MontiRed-LOV2dark
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LOV2: C450A, L514K, G528A, L531E, N538E

    Promoter

    CAG

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FLAG-SLC30A10-D248A

    Plasmid

    #82346

    Purpose

    Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D248A

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFLAG-mISG15-4EHP

    Plasmid

    #17354

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    mISG15-4EHP (EIF4E2 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

40. myc-hIFITM3-F63Q

    Plasmid

    #104363

    Purpose

    Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated the following amino acid: F63Q

    Promoter

    CMV

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge