Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 20,500 results for: cas9 genes

Showing: 7321 - 7360 of 20500 results

  4. pCRISPEY-Z3-Kan

    Plasmid
    #232102
    Purpose
    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    232102-plasmid-map-sequence-id-462314

    Enlarge

  5. pCRISPEY-Z3-Hyg

    Plasmid
    #232101
    Purpose
    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232101-plasmid-map-sequence-id-462305

    Enlarge

  6. pEF1Cas-LT1

    Plasmid
    #210556
    Purpose
    Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    pMpEF1::Cas9::PEA3AT::U6p::AAVS1gRNA::EF1p::LT1::Hygromycin
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    210556-plasmid-map-sequence-id-426215

    Enlarge

  7. pCitro-15a

    Plasmid
    #202033
    Purpose
    p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter
    Depositor
    David Edgell
    Article
    Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-
    Insert
    conjugative system from a Citrobacter spp.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202033-plasmid-map-sequence-id-403993

    Enlarge

  12. pSB1C3-FeGFP

    Plasmid
    #138261
    Purpose
    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138261-plasmid-map-sequence-id-273630

    Enlarge

  13. pJM108

    Plasmid
    #134160
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    KMLL, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134160-plasmid-map-sequence-id-271380

    Enlarge

  14. pJM107

    Plasmid
    #134159
    Purpose
    Retroviral plasmid expressing GFP - Nde1
    Depositor
    Iain Cheeseman
    Article
    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2
    Insert
    Nde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    134159-plasmid-map-sequence-id-271374

    Enlarge

  18. AP1298‐1

    Plasmid
    #87248
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::recoded gtbp‐1(8/27bp, every 3bp)::PH::gtbp‐1 (flanking sequence)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87248-plasmid-map-sequence-id-173231

    Enlarge

  23. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

    Enlarge

  24. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

    Enlarge

  25. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

    Enlarge

  26. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid
    #114009
    Purpose
    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
    Depositor
    Pavel Volchkov
    Article
    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126
    Inserts
    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    -, CMV, and endogenous promoter (chicken GAPDH in…
    Available since
    Jan. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    114009-plasmid-map-sequence-id-261238

    Enlarge

  27. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80436-plasmid-map-sequence-id-151445

    Enlarge

  28. pSc2

    Plasmid
    #80437
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80437-plasmid-map-sequence-id-151450

    Enlarge

  29. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107999-plasmid-map-sequence-id-217151

    Enlarge

  31. pP[RS3]3'M

    Plasmid
    #53552
    Purpose
    for an easy screen of TALEN- and CRISPR/Cas9- mediated mutagenesis in Drosophila
    Depositor
    Renjie Jiao
    Article
    Yu et al Biol Open. 2014 Apr 15;3(4):271-80. doi:
    Insert
    AscI and MluI restriction enzyme sites
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    same pP[RS3]
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 7321 - 7360 of 20500 results