164,298 results

1. MAC-tag-N

   Plasmid

   #108078

   Purpose

   Gateway compatible destination vectors containing StrepIII/HA/BioID

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CCDB (ccdB(letD) )
   

   Use

   Tags

   StrepIII-HA-BirA*

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-MTS-jAspSnFR3-mRuby3

   Plasmid

   #203492

   Purpose

   Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag, Mitochondrial localization signal, and m…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-UbC-Tet1v4-dCas9

   Plasmid

   #232180

   Purpose

   Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGIG

   Plasmid

   #11159

   Purpose

   Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATetO 6XHis-Phusion

   Plasmid

   #206210

   Purpose

   Inducible expression of DNA polymerase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

   Insert

   Phusion DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pRK5-A3-HA-ubiquitin

   Plasmid

   #220145

   Purpose

   Expresses A3-Ub for E3 substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   A3-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX302 Luciferase-V5 puro

    Plasmid

    #47553

    Purpose

    Lentiviral expression of V5-tagged Luciferase in mammalian cells

    Depositor

    Kevin Janes

    Article

    Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-FGFR3-S249C

    Plasmid

    #116386

    Purpose

    Lentiviral expression of FGFR3 S249C

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S249C

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS-lpp0.2-K64A

    Plasmid

    #201789

    Purpose

    mutant of PadR; transcriptional regulator can respond to p-coumaric acid

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    PadR (padR Bacillus subtilis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K64A

    Promoter

    Available since

    July 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEGFP GR

    Plasmid

    #47504

    Purpose

    Expresses glucocorticoid receptor in mammalian cells

    Depositor

    Alice Wong

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAIDA1

    Plasmid

    #79180

    Purpose

    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

    Depositor

    Gen Larsson

    Article

    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

    Insert

    Adhesin Involved in Diffuse Adherence
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pToneDest

    Plasmid

    #67691

    Purpose

    attR4-attR3 Destination cassette in Tol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas13d-dsRBD-APEX2

    Plasmid

    #154939

    Purpose

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

    Plasmid

    #209727

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Promoter

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-His:dCas9:nls:10P3

    Plasmid

    #135982

    Purpose

    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    dCas9 fused to 10 copies of the P3 coiled-coil
    

    Use

    CRISPR

    Tags

    His

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

    Plasmid

    #221359

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. Nb-gp41-Halo (MoonTag-Nb-Halo)

    Plasmid

    #128603

    Purpose

    Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and Halo

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFastBac-CHD7

    Plasmid

    #170141

    Purpose

    Expresses CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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