167,785 results

1. hZMPSTE24-3FLAG_pQCXIP

   Plasmid

   #89759

   Purpose

   Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

   Depositor

   Martin Dorf

   Article

   Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

   Insert

   Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
   

   Use

   Retroviral; Bicistronic expression: puromycin re…

   Tags

   9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

   Expression

   Mammalian

   Mutation

   Y253H in ZMPSTE24 (see depositor comments below)

   Promoter

   CMV

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anep8_Cas9

   Plasmid

   #117169

   Purpose

   Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

   Depositor

   Adrian Tsang

   Article

   Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Filamentous fungi e…

   Promoter

   Pki

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

   Plasmid

   #234950

   Purpose

   Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LTN1_pCSdest

   Plasmid

   #53855

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   LTN1 (LTN1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. SPECS library

   Pooled Library

   #127842

   Purpose

   For performing high-throughput cell state-specific synthetic promoter screening

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Aug. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pCggRO-reporter

   Plasmid

   #124582

   Purpose

   Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

   Depositor

   Matthias Heinemann

   Article

   Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

   Insert

   CggR promoter element
   

   Expression

   Yeast

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GAL2-Tango

   Plasmid

   #66289

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GAL2 (GALR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Sybody Generation Toolbox

   Plasmid Kit

   #1000000160

   Purpose

   The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

   Depositor

   Markus Seeger

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pDEST-EGFP-TARG1

    Plasmid

    #172586

    Purpose

    For transient expression of N-terminal GFP-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-HAT1_AG

    Plasmid

    #217113

    Purpose

    Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HAT1_AG transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1-FOXM1b

    Plasmid

    #68811

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1b (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-Neurexin-1-Beta [N170A/1.1R]

    Plasmid

    #114530

    Purpose

    Mammalian Expression Plasmid of anti-Neurexin-1-Beta (Human). Derived from hybridoma N170A/1.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neurexin-1-Beta (Homo sapiens) recombinant mouse monoclonal antibody (NRXN1 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Marisa Brini, Tito Calì

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. tdTomato-CD151-7

    Plasmid

    #58079

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CD151 (CD151 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TJP1_PDZ_2

    Plasmid

    #103910

    Purpose

    Protein expression and purification of ZO1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-alphaMHC 3300bp

    Plasmid

    #51934

    Purpose

    luciferase reporter for rat alpha-MHC promoter

    Depositor

    Jeffery Molkentin

    Insert

    alpha MHC (Myh6 Rat)
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    -2930 to +410 promoter region

    Promoter

    alpha MHC

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-PARP1_D770A-mCherry

    Plasmid

    #192260

    Purpose

    Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_D770A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D770A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PARP1_R878A-mCherry

    Plasmid

    #192261

    Purpose

    Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_R878A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R878A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP)

    Plasmid

    #215003

    Purpose

    Shuttling vector for a Lysophagy reporter

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Gateway

    Tags

    HaloTag(7)-mGFP

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX-GP-2-KDM4A double Tudor

    Plasmid

    #59699

    Purpose

    Contains histone modification interacting domain (KDM4A double Tudor)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    KDM4A double Tudor (KDM4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLEX305_3xFLAG_RELA1

    Plasmid

    #220324

    Purpose

    Expresses full length human RELA with an N-terminal 3xFLAG tag

    Depositor

    Jonathan Brown

    Insert

    RELA (RELA Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    hPGK

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. OPRM1-DuET

    Plasmid

    #213361

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRM1 (OPRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. fluoPEER-GFP>BFP

    Plasmid

    #185477

    Purpose

    Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP-Y66-editing-site
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. TJP1_PDZ_1

    Plasmid

    #103909

    Purpose

    Protein expression and purification of ZO1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

    Plasmid

    #166951

    Purpose

    Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ-Y527A (SFPQ Human)
    

    Use

    Lentiviral

    Tags

    GFP and mCherry

    Mutation

    SFPQ-Y527A

    Promoter

    eF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP.N3-SLC35A1-GFP

    Plasmid

    #186281

    Purpose

    transient expression of C-terminal GFP tagged SLC35A1 isoform a in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

    Insert

    SLC35A1 (SLC35A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDEST-V1-ORF

    Plasmid

    #73635

    Purpose

    Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. Tad-CBE(ACC2)

    Plasmid

    #235061

    Purpose

    Expresses Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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