We narrowed to 14,310 results for: eng

Showing: 361 - 380 of 14310 results

Results per page:

Sort results by:

pLL7.0: tgRFPt-SSPB R73Q

Plasmid

#60416

Purpose

Mammalian Expression of tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

Enlarge
pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Publication

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Publication

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pLenti6-MK1-EHMT2-V5

Plasmid

#31113

Depositor

Bernard Futscher

Insert

euchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Stop codon mutated to Ala

Available since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Expression

Bacterial

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pLCNICK

Plasmid

#84653

Purpose

Genome editing for Lactobacillus casei Lc2W

Depositor

Sheng Yang

Publication

Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

Inserts

Cas9 nickase
sgRNA
homology arms of LC2W_2179

Use

CRISPR

Mutation

D10A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pMevT

Plasmid

#17815

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pTALdNC-AAVS1_T1

Plasmid

#80495

Purpose

AAVS1 TALEN expression vector (Left)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Left)

Use

Gateway Cloning

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pTALdNC-AAVS1_T2

Plasmid

#80496

Purpose

AAVS1 TALEN expression vector (Right)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 TALEN (Right)

Use

Gateway Cloning

Promoter

none

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pX330 Pten

Plasmid

#59909

Purpose

pX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoter

Depositor

Tyler Jacks

Publication

Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

Insert

sgRNA targeting Pten (Pten Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
T7-RelA

Plasmid

#21984

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

NFkB p65 subunit (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

For more details for this expression construct,…

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

nanos

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

Enlarge
cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Publication

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Publication

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

Enlarge
pSGAb-km

Plasmid

#121999

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Publication

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Publication

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pcDNA3-Lyso-TORCAR

Plasmid

#64929

Purpose

Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Publication

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

LAMP1-TORCAR (LAMP1 Human, Synthetic)

Tags

Cerulean, Full-length LAMP1, and YPet

Expression

Mammalian

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge