We narrowed to 22,871 results for: ESC

1. pEB1-mCerulean

   Plasmid

   #103968

   Purpose

   Plasmid encoding mCerulean

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCerulean
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, S65T, …

   Promoter

   ProC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mPA-GFP-Farnesyl-5

   Plasmid

   #57133

   Purpose

   Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_HaloTag7_LamB1

   Plasmid

   #175528

   Purpose

   Expression of HaloTag7 at the nuclear lamina of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag7-LamB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEos3.2-N1

   Plasmid

   #54525

   Purpose

   Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. mEos3.2-C1

   Plasmid

   #54550

   Purpose

   Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson, Tao Xu

   Article

   Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

   Type

   Empty backbone

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pTKDP-dhfr

   Plasmid

   #71323

   Purpose

   Donor plasmid - trimethoprim resistant donor cassette

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKeratin-LSSmOrange

   Plasmid

   #37138

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange
   

   Use

   Tags

   human cytokeratin 18

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. phage-ubc-nls-ha-tdMCP-gfp

   Plasmid

   #40649

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdMCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUGW-pHRed

    Plasmid

    #65742

    Purpose

    Lentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter

    Depositor

    Mathew Tantama

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    pHRed
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-puroeGFP

    Plasmid

    #177922

    Purpose

    Constitutively expresses eGFP with puromycin selection

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2+-eGFP

    Plasmid

    #221710

    Purpose

    Expression of eGFP in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    eGFP
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCytERM-mNeptune2.5-N1

    Plasmid

    #197194

    Purpose

    Expresses the fusion protein of CytERM-mNeptune2.5 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    CytERM-mNeptune2.5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HRE-dUnOHR

    Plasmid

    #201714

    Purpose

    Hypoxia responsive promoter driving UnaG and mOrange

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR1a-CRISPRoffv2.1

    Plasmid

    #207174

    Purpose

    Gateway Cloning Vector for CRISPRoffv2.1

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPPAT-mCherry

    Plasmid

    #167162

    Purpose

    Expresses human PPAT-mCherry fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    Pedley et al Methods Mol Biol. 2018;1764:279-289. doi

    Insert

    PPAT (PPAT Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTurquoise2-MapTau

    Plasmid

    #137807

    Purpose

    Visualization of microtubules

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    MapTau
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMF19

    Plasmid

    #113662

    Purpose

    Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

    Depositor

    Miguel Valvano

    Article

    Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

    Insert

    wbbL
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ptac

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMO9075

    Plasmid

    #117481

    Purpose

    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR

    Depositor

    Judy Wall

    Article

    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1

    Insert

    Aph(3')-IIa promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aph(3')-IIa promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Ub-R-GFP

    Plasmid

    #11939

    Depositor

    Nico Dantuma

    Article

    Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

    Insert

    Ub-R-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ubiquitin fused to N-terminus of GFP. Arginine at…

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMVlux-cp157Venus

    Plasmid

    #197584

    Purpose

    BRET lux luciferase with cp157Venus for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    human codon-optimized Lux-cp157Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJYDC1

    Plasmid

    #162458

    Purpose

    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    HA tag, HDEL sequence, eUnaG2 fluorescent protein…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST-periHisMBP

    Plasmid

    #11086

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and MBP signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTAKN2_J23119-sgRNA

    Plasmid

    #108552

    Purpose

    Bacterial sgRNA expression vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    2x Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pH2B-miRFP680-N1

    Plasmid

    #197229

    Purpose

    Expresses the fusion protein of H2B-miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    H2B-miRFP680
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEB1-mVenus NB

    Plasmid

    #103986

    Purpose

    Plasmid encoding mVenus NB

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus NB
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTH101

    Plasmid

    #84459

    Purpose

    pJB38-NWMN29-30 + SarA_P1-DsRed-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-DsRED from pTH1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Use

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCytERM-BDFP1.8-N1

    Plasmid

    #197186

    Purpose

    Expresses the fusion protein of CytERM-BDFP1.8 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    CytERM-BDFP1.8-
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRN110

    Plasmid

    #84462

    Purpose

    pJB38-NWMN29-30 + SarA_P1-mKate2-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mKate2 from pRN11
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJYDN

    Plasmid

    #162450

    Purpose

    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Andrew Ellington, Ichiro Matsumura

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMVMagFRET-1

    Plasmid

    #50742

    Purpose

    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GPI-mNeonGreen(2x)

    Plasmid

    #197913

    Purpose

    Homo-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    glycosylphosphatidylinositol-mNeonGreen-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mOrange2-N1

    Plasmid

    #54568

    Purpose

    Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLRD13

    Plasmid

    #208265

    Purpose

    Production of ent-cassa-12,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    osKSL7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge