167,825 results

1. ABE8.20-NG

   Plasmid

   #226857

   Purpose

   Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-NG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOS008: GCaMP6F calcium sensor (cytosolic)

   Plasmid

   #163045

   Purpose

   GCaMP6F calcium sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   GCaMP6F
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100854

   Purpose

   AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR1k-J23107-GFPuv

   Plasmid

   #106389

   Purpose

   pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEG BacMam N term His8 eGFP 3C

   Plasmid

   #160684

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   His8 eGFP 3C

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-mEGFP(A206K)-paCaMKII(K42M)

   Plasmid

   #165440

   Purpose

   Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(K42M)
   

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPC1 His6 EGFP

   Plasmid

   #53521

   Depositor

   Matthew Scott

   Article

   Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

   Insert

   NPC1
   

   Tags

   EGFP and His6

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_5-HT1.0 (AAV9)

    Viral Prep

    #140552-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_5-HT1.0 (#140552). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_5-HT1.0 plasmid DNA. Synapsin-driven expression of GRAB_5-HT1.0 sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

    Promoter

    Syn

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

11. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCbs FlagOmomyc

    Plasmid

    #113168

    Purpose

    Expression of FLAG tagged Omomyc in mammalian cells

    Depositor

    Sergio Nasi

    Article

    Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

    Insert

    Omomyc (MYC Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E57T, E64I, R70Q, R71N

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE048-SiT-Cas12a

    Plasmid

    #128124

    Purpose

    Express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCmini-iCAP-PHP.B

    Plasmid

    #103002

    Purpose

    non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC1-mitoRexYFP

    Plasmid

    #60246

    Purpose

    Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    mitoRexYFP
    

    Tags

    Mitochondrial targeting signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW335c3

    Plasmid

    #237322

    Purpose

    expresses A. thaliana RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    A. thaliana RbcL (rbcL Mustard Weed)
    A. thaliana RbcS-6xHis
    

    Tags

    6xHis and None

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV1)

    Viral Prep

    #162381-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    CAG

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

21. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. mCherry-Tubulin-C-18

    Plasmid

    #55148

    Purpose

    Localization: Microtubules, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLCKO

    Plasmid

    #73311

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV1)

    Viral Prep

    #84446-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Promoter

    CAG

    Tags

    tdTomato

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

26. pDONR223-MAP2K1

    Plasmid

    #23406

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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27. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3-HA-VprBP

    Plasmid

    #133586

    Purpose

    Expresses human VprBP wildtype in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    Vpr (HIV-1) binding protein (DCAF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Wildtype human VprBP (Dcaf1)

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pOTTC407 - pAAV EF1a eGFP

    Plasmid

    #60058

    Purpose

    An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    enhanced Green Fluorescent Protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23105 and tet

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. Klc1-GFP

    Plasmid

    #186617

    Purpose

    Encodes Kinesin Light Chain 1 fused to GFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Light Chain 1 (KLC1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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33. EGFP-GRAM1b

    Plasmid

    #211494

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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34. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)

    Viral Prep

    #112005-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Tags

    mRuby3

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA3/HF-CDC50A

    Plasmid

    #209223

    Purpose

    Mammalian expression of CDC50A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    CDC50A (TMEM30A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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36. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

    Plasmid

    #162377

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWZL Neo Myr Flag SRPK2

    Plasmid

    #20525

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    SRPK2 (SRPK2 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

38. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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39. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-HA-hIFITM3

    Plasmid

    #58397

    Purpose

    Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge