20,445 results

1. NPY-td-Orange2

   Plasmid

   #83497

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   tandem mOrange2

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-RSV-tdTomato-2A-GCaMP6s

   Plasmid

   #80316

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Darrell Kotton

   Article

   Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

   Inserts

   tdTomato
   GCaMP6s
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   RSV

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1(-) mouse Egr2

   Plasmid

   #107997

   Purpose

   Expresses mouse Egr2 in mammalian cells

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   Egr2 (Egr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PH-Akt(R25C)-GFP

   Plasmid

   #51466

   Purpose

   Non-PIP3 binding control for PH-Btk-GFP

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R25C

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pYPQ131B

   Plasmid

   #69281

   Purpose

   Golden Gate entry vector; 1st gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_ARNTL_WT

   Plasmid

   #82189

   Purpose

   Gateway Donor vector containing ARNTL , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ARNTL (BMAL1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST26-C-FLAG

   Plasmid

   #79275

   Purpose

   Destination vector containing a C-terminal FLAG tag

   Depositor

   Rita Shiang

   Article

   Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

   Insert

   FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pAAV-CAG-mGreenLantern

   Plasmid

   #164469

   Purpose

   Constitutive CAG-driven expression of mGreenLantern in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CAG

   Available since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-FLIP-puro-dsRed-GFP-miRNA

    Plasmid

    #32704

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    emGFP-miRNA
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL410_INS421

    Plasmid

    #49057

    Purpose

    Expresses luciferase under control of human insulin gene promoter

    Depositor

    Kevin Ferreri

    Article

    Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

    Insert

    human INS promoter (INS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INS promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Plasmid

    #163077

    Purpose

    Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Peredox
    

    Use

    Gateway entry vector

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRD215

    Plasmid

    #167555

    Purpose

    Backbone plasmid to pRMA154

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

    Type

    Empty backbone

    Use

    Broad host range mobilisable

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FlagETR3 (CELF2)

    Plasmid

    #96900

    Purpose

    expression of Flag-tagged human CELF2 (aka CUGBP2)

    Depositor

    Thomas Cooper

    Article

    Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

    Insert

    human CELF2 cDNA (CELF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage UbiC scAB-GFP

    Plasmid

    #104998

    Purpose

    Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    single chain Antibody against GCN4
    

    Use

    Lentiviral

    Tags

    GB1, HA tag, and sfGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMW#2

    Plasmid

    #13349

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    HIS3 reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE2-SpRY-P2A-GFP

    Plasmid

    #159981

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY-P2A-GFP
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSNR52-sgTEF1

    Plasmid

    #46922

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgTEF1 promoter (TEF1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHLmMBP-8

    Plasmid

    #72347

    Purpose

    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG_mRuby2_smFP OLLAS

    Plasmid

    #59761

    Purpose

    encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_OLLAS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmAmetrine-DEVD-tdTomato

    Plasmid

    #18879

    Purpose

    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

    Depositor

    Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Insert

    mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLX317-QKI

    Plasmid

    #115441

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    QKI (QKI Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCIRCNpu

    Plasmid

    #74226

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Npu DnaE C-intein and Npu DnaE N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHRm-GFP(1-10)-VP64-NLS

    Plasmid

    #70228

    Purpose

    Expresses GFP(1-10)-VP64-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP(1-10)-VP64-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.GFAP.iGluSnFR3.v857.GPI

    Plasmid

    #178338

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4-tat

    Plasmid

    #22502

    Depositor

    Sergey Kasparov

    Article

    Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

    Insert

    Transactivator of Transcription
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS-memb-mCherry

    Plasmid

    #53750

    Purpose

    Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    2x membrane linker
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (AAV5)

    Viral Prep

    #106180-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.hSynap-FLEX.SF-iGluSnFR.A184S (#106180). In addition to the viral particles, you will also receive purified pAAV.hSynap-FLEX.SF-iGluSnFR.A184S plasmid DNA. Synapsin-driven, Cre-dependent expression of glutamate sensor iGluSnFR (high affinity). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Promoter

    Syn

    Tags

    None

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

31. pFUW-tetO-Bcl11a

    Plasmid

    #139809

    Purpose

    doxycycline-inducible expression of human Bcl11a in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL11a (BCL11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. m6A-Tracer-NES

    Plasmid

    #159607

    Purpose

    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    m6A-Tracer-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223-ROR2

    Plasmid

    #23927

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ROR2 (ROR2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA AXL

    Plasmid

    #105932

    Purpose

    Mammalian expression of myc-His tagged AXL

    Depositor

    Rosa Marina Melillo

    Article

    Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

    Insert

    AXL (AXL Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Promoter

    tet-inducible CMV

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid

    #114070

    Purpose

    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-EGFP-P4M-SidMx2

    Plasmid

    #136997

    Purpose

    Lentiviral EGFP lipid sensor for PI(4)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-P4M-SidMx2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EF1a_MYCN_P2A_Hygro_Barcode

    Plasmid

    #120463

    Purpose

    Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.3_SARS2_XBB.1.5

    Plasmid

    #196585

    Purpose

    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G252V, F486P on the basis of XBB

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSBbi-GN

    Plasmid

    #60517

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDL-hu FceRI alpha

    Plasmid

    #8365

    Depositor

    Jean Kinet

    Article

    Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

    Insert

    human Fc epsilon RI alpha cDNA (FCER1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

44. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    gfap

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Flag-HA-USP14

    Plasmid

    #22569

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP14 (USP14 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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46. PB-gRNA-Puro

    Plasmid

    #121121

    Purpose

    Expression of a gRNA together with a Puromycin resistance

    Depositor

    Pablo Navarro

    Article

    Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA5/FRT/TO-GRB2-GFP

    Plasmid

    #86873

    Purpose

    To express GRB2 with a GFP tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. Perturb-seq GBC library

    Pooled Library

    #85968

    Purpose

    Perturb-seq GBC library

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

49. p23-NLS-RfxCas13d-mcherry-NLS-Flag

    Plasmid

    #165077

    Purpose

    overexpression of RfxCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RfxCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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