167,825 results
-
Plasmid#179464PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSynDepositorHas ServiceAAV9InsertJEDI-2P
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
TXNRD1
Plasmid#38863PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ERT2-iCre-ERT2
Plasmid#178059PurposeConditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.DepositorInsertERT2-iCre-ERT2
UseAAVExpressionMammalianPromoterHuman synapsin 1 (Syn) promoterAvailable SinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xFLAG-TurboID-MCS
Plasmid#186813PurposeCAG promoter-driven expression of TurboID-fused protein (N terminus)DepositorTypeEmpty backboneTags3xFLAG-TurboIDExpressionMammalianPromoterCAG promoterAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-dPspCas13b-NES-mCherry
Plasmid#233609PurposeExpression of catalytically-inactive PspCas13b in mammalian cellsDepositorInsertdPspCas13b-NES-mCherry
UseCRISPRTags3xFlag and mCherryMutationH133A/H1058AAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL10-IRES-mCherry-PGK-BFP
Plasmid#223209PurposeLentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
-5.5kb-UCP1-GFP
Plasmid#104585PurposeReporter assay to monitor the transcription activity of the UCP1 promoter by GFP expressionDepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)
Plasmid#220611PurposeNeuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.DepositorInsertFLAG-KORD-P2A-3x HA-hM3D(Gq)
UseAAV and Cre/LoxTagsFLAG; ArgiNLSExpressionMammalianPromoterhSynAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)
Viral Prep#137148-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre and Flp-dependent)Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-ch-TOG-His6
Plasmid#69108PurposeFor bacterial expression of recombinant human ch-TOG tagged with MBP and His.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
TagsHis6 and MBPExpressionBacterialAvailable SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-neo (1795)
Plasmid#41000DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmBanana-Hyg-CTCF-CtermNES-N1
Plasmid#131851PurposeExpresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cellsDepositorInsertCTCF (CTCF Human)
TagsmBananaExpressionMammalianMutationthe C-terminus of CTCF is linked to HIV-Rev prote…Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK18sB
Plasmid#177838PurposePlasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.DepositorTypeEmpty backboneUseBacterial gene replacementAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hIFITM1
Plasmid#58399PurposeExpressed human IFITM1 with an N-terminal HA tag in mammalian cellsDepositorAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-pur H-2Kb
Plasmid#111623PurposeSleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I alleleDepositorInsertH2Kb
ExpressionMammalianPromoterEF1alphaAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_EGFP
Plasmid#25899PurposeGateway entry vector containing EGFP.DepositorInsertEGFP
UseGateway entry vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_SEPT9 i4
Plasmid#71617Purposemammalian expression of human SEPT9 i4 with a GFP fusionDepositorAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CycK-GFP (1-270AA) in Cilantro (Codon optimized)
Plasmid#169930PurposeOverexpression of 1-270AA CycK-GFP fusionDepositorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK2
Plasmid#101296PurposeMammalian expression of Rock2-GFPDepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA
Plasmid#156436PurposeFor transient expression of human PDS5B tagged with mKate2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_EML4_ALK
Plasmid#205809PurposeExpress mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-EGFP
Plasmid#80605PurposeMammalian expression vector that expresses HIV-1 GAG with EGFP fused to itDepositorInsertEGFP
ExpressionMammalianPromoterSRalphaAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pKLV-U6gRNA(BbsI)-PGKpuro2ABFP
Plasmid#50946PurposeAn empty gRNA expression vectorDepositorInsertNone
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRDA_478
Plasmid#179096PurposeBE Cas expressionDepositorInsertBE3.9 (SpG)
UseCRISPR and LentiviralAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
ExpressionBacterialPromoterTac PromoterAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE38
Plasmid#90378PurposeRELB - NFkB gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterMAFAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213940-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorTagsdTomatoAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/v5-DEST hGlut4
Plasmid#18087DepositorInsertGlut4 (SLC2A4 Human)
ExpressionMammalianAvailable SinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
NYESO beta/alpha into TRAC HDRT Source (pTR 169)
Plasmid#112021PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alphaDepositorInsertNYESO beta/alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyAvailable SinceSept. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_3xFlagEzh2 WT
Plasmid#173717PurposeWT EZH2 expression plasmid with 3XFLAG tagDepositorAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen
Plasmid#205022Purposemini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-JMJD2B
Plasmid#24181PurposeMammalian expression of human JMJD2B with HA tagDepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast tBID-flag
Plasmid#211533PurposeExpresses truncated BID upon doxycycline addition to trigger apoptosisDepositorInserttBID-flag (BID Human)
UseRetroviralTagsFLAGExpressionMammalianMutationTruncated (CASP8) form (p15)Available SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK025
Plasmid#236572PurposeCloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into ISYmu1 plant expression vectorDepositorTypeEmpty backboneExpressionPlantAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)
Viral Prep#112677-AAV9PurposeReady-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only