175,750 results
-
Plasmid#180375PurposePseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike proteinDepositorInsertSpike (omicron BA.1 variant) (S SARS-CoV-2)
UseLentiviral and RetroviralExpressionMammalianMutationc terminal D18PromoterCMVAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS9
Plasmid#111765PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable SinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
Plasmid#207159PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG1
ExpressionMammalianMutationN-terminally tagged with LSS-mKATE2Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S129A
Plasmid#36066DepositorAvailable SinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET14b – lambda exonuclease
Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
TagsHis6 followed by a thrombin siteExpressionBacterialPromoterT7Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_IRES_GFP_CD16a
Plasmid#196189PurposeRetroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.DepositorInsertCD16a (FCGR3A Human)
UseRetroviralExpressionMammalianMutationchanged Phenylalanine 158 to ValinePromoterLTRAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NlucP
Plasmid#162595PurposeC-terminally PEST-tagged NanoLuc under TRE3G promoterDepositorInsertNanoLuc-PEST
TagsPESTExpressionMammalianPromoterTRE3G promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
SQ171/pCSacB/ptRNA100
Bacterial Strain#155206PurposeSQ171 strain supported by pCSacB and ptRNA100 plasmidsDepositorBacterial ResistanceKanamycinAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRExpressionMammalianAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
EIF2AK3-NanoLuc Fusion Vector
Plasmid#237169PurposeExpress EIF2AK3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GluRS-His
Plasmid#124109PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 PLIN2
Plasmid#202196PurposepX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2DepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B
Plasmid#172203PurposeExpresses KIF5B tagged with eGFP in mammalian cellsDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVMutationNAPromoterCAGAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Golgi-TurboID
Plasmid#200959PurposeTransiently expresses golgi localized TurboID with HA tagsDepositorInsertGolgi-TurboID
TagsHAExpressionMammalianPromoterCMVAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposePlasmids for Golden Gate-based assembly of cellular circuits for use in mammalian cell.DepositorApplicationCloning and Synthetic BiologyVector TypeMammalian ExpressionCloning TypeGolden GateAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synapto-pHluorin
Plasmid#168510PurposeExpression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.DepositorAvailable SinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-nTVMVP-AP4-tevs-P3-tevs-cTVMVPmut
Plasmid#179660PurposeCytosolic TEVP-inducible caged-nTVMVPDepositorInsertCaged-nTVMVP
ExpressionMammalianAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-nTVMVPmut-tevs-AP4-tevs-P3-cTVMVP
Plasmid#179661PurposeCytosolic TEVP-inducible caged-cTVMVPDepositorInsertCaged-cTVMVP
ExpressionMammalianAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-ISceID44A
Plasmid#59424PurposeExpresses a catalytically inactive form of the endonuclease ISceIDepositorInsertendonuclease ISceI mutant (I-SceI )
TagsHA tagExpressionMammalianMutationchanged aspartate 44 to alanine (D44A)Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-GFP-IRF3
Plasmid#127663PurposeOver-express EGFP-IRF3DepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCBh; U6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-eBFP2
Plasmid#102640PurposeIn vitro expression of eBFP2 from the T7 promoterDepositorInserteBFP2
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-EGFP (AAV8)
Viral Prep#50469-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEGFPAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y530F
Plasmid#140318PurposeExpresses HA-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-HA-ORF-T2A-Puro
Plasmid#242698Purposegene expressionDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMammalianAvailable SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-Adcy3-3xFlag
Plasmid#245989PurposeExpress 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceOct. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
pLM-fSV2A
Plasmid#27512DepositorInsertOCT4_T2A_KLF4_P2A_cMYC_E2A_Sox2
UseLentiviralAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hAIM2
Plasmid#51542PurposeExpress GFP-tagged hAIM2 in mammalian cellsDepositorAvailable SinceMarch 19, 2014AvailabilityAcademic Institutions and Nonprofits only