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Plasmid#167666PurposeENTRY vector for gRNA4 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-Zim3-MeCP2
Plasmid#220143Purposedoxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repressionDepositorInsertdCas9-Zim3-MeCP2
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTre-3GAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBW001
Plasmid#131529PurposeVector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactionsDepositorArticleInsertMu-BsaI-KanR transposon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6d:sgRNA#5
Plasmid#64249Purposeexpresses sgRNA under U6d promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-flpE-puro
Plasmid#20733PurposeMammalian expression of FLPe recombinase from the highly-expressed CAGGS promoterDepositorInsertFLPe recombinase
UseFlp/frtTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mGreenLantern
Plasmid#164469PurposeConstitutive CAG-driven expression of mGreenLantern in animals using AAVDepositorInsertmGreenLantern
UseAAVTagsExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCAGAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_EF1aUBA3_IRES_Puro
Plasmid#235660Purposehuman UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)DepositorInsertUBA3 (UBA3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
BL21∆serB
Bacterial Strain#34929DepositorBacterial ResistanceNoneAvailable sinceFeb. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Drp1K38A
Plasmid#45161DepositorInsertDrp1 (DNM1L Human)
UseTagsHA (AYPYDVPDY)ExpressionMammalianMutationK38APromoterCMVAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[30Q]
Plasmid#33236DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains a stretch of 29 amino acids (27 Q…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-puro-N3FLAG-MYB-FL
Plasmid#105596Purposeretrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistanceDepositorInsertFull length MYB (mouse) (Myb Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiP14870 - pAAV-AiE0452h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4870)
Plasmid#224186PurposeAiE0452h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNsInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0725
Plasmid#143071PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKB2 (NFKB2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf1-16
Plasmid#198271PurposeUnmodified pPVC; contains WT PVC structural/accessory genesDepositorInsertPVCpnf1-16
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Puro_siKD
Plasmid#86695PurposeTetracycline inducible expression of shRNA targeted to AAVS1 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre (AAV8)
Viral Prep#121675-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsStrepII-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-Wasabi
Plasmid#86117PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38DepositorInsertsTcI38
mWasabi
UseTags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterpR/pL and pR/pL, LacIAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEK3394
Plasmid#234078PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInserttspurple
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
8061-E33
Plasmid#234270PurposeGateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)DepositorInsertSmad3 (Smad3 Mouse)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-Jph3 WPRE
Plasmid#236238PurposeAAV expression of human Junctophilin3 N-terminally tagged with HaloTagDepositorInsertJunctophilin 3 (JPH3 Human)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3238
Plasmid#234092PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfCherry2
UseTagsExpressionBacterialMutationE117Q/T127I/G219APromoterpJ23100-BsaIAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3228
Plasmid#234090PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertaeBlue
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3227
Plasmid#234089PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS CMV GAG
Plasmid#234992PurposeFor production of Viral like particles (VLPs) by expressing the MLV GAG protein in mammalian cellsDepositorInsertGAG
UseTagsExpressionMammalianMutationDeleted polymerase coding sequencePromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2EYI
Plasmid#234173PurposeHeterologous protein expression of alpha-actinin-1 in Escherichia coliDepositorInsert2EYI (ACTN1 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdest METTL1-WT Neo
Plasmid#223058PurposeMETTL1 gene expression in mammalian cellsDepositorInsertMETTL1 (METTL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC081: pAAV.CMV.mCherry
Plasmid#205744PurposeAAV vector for expressing CMV-driven mCherry reporter gene.DepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
NO lux Sensor
Plasmid#199790PurposeSynthetic gene circuitDepositorInsertNO lux Sensor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SUN2 mApple
Plasmid#187679PurposeIn vivo visualization of SUN2DepositorInsertSUN2 (Sun2 Mouse)
UseTagsmAppleExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-DLX1 [L43/40R]
Plasmid#128633PurposeMammalian Expression Plasmid of anti-DLX1 (Human). Derived from hybridoma L43/40.DepositorInsertanti-DLX1 (Human) recombinant mouse monoclonal antibody (DLX1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-TID-HA3-HIS3MX6
Plasmid#227981PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-PCNA
Plasmid#215117PurposeExpresses mCherry-PCNA in mammalian cells from a lentiviral vector.DepositorInsertPCNA (PCNA Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only