We narrowed to 18,387 results for: Emb;
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Plasmid#175027PurposeExpresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (H85E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG)PromoterCMVAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj14
Plasmid#173144PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj14 (kcnj14 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1a
Plasmid#164956PurposeZebrafish kcnn1a gene CDSDepositorInsertkcnn1a (kcnn1a Zebrafish)
UsePcr cloning vectorAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r
Plasmid#162967PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteinsDepositorInsertDual Strep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Plasmid#162958PurposeGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteinsDepositorInserttriple FLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtUBQ10 (GB2478)
Plasmid#160594PurposeUBQ10 promoter from A. thaliana for constitutive expression in plant systems.DepositorInsertAtUBQ10
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 nickase D10A Cas9 (GB1690)
Plasmid#160587PurposeNickase D10A Cas9 for Nt and Ct fusion.DepositorInsertnickase D10A Cas9
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn3
Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorInsertkcnn3 (kcnn3 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn4
Plasmid#164960PurposeZebrafish kcnn4 gene CDSDepositorInsertkcnn4 (kcnn4 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
TagsHis6-tagExpressionMammalianPromoterChicken beta-actin promoterAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(G124I)
Plasmid#130672PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
TagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, G124IAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV
Plasmid#137118PurposeProtease Switch Clone ID: 2-B3DepositorInsertAI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV
UseSynthetic BiologyAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-GFP-ePDZ-CAAX
Plasmid#135603PurposeExpression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ-CAAX
UseIn vitro transcription (mrna synthesis)TagsCAAX, EGFP, and ePDZPromoterUnknownAvailable SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTransgenesis p3 sv40 minimal promoter:: Katushka RFP
Plasmid#131731PurposeTransposon based transgenesisDepositorInsertSV40MinPromoter
UseXenopus expressionAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
Plasmid#127636PurposeEncodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)DepositorInsertmTurquoiuse2-Tat, YPet-PCP
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsHis and mCherryExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pESC/CURT_fluoB
Plasmid#117997PurposeExpression of CURT_fluoB controlled by the Gal1 promoterDepositorInsertCURT_fluoB
UseYeast/bacteria binary vectorExpressionYeastPromoterGal1Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TMK1 X035_pECIA14
Plasmid#114871PurposePrey vector TMK1 X035_pECIA14 should be used with bait vector TMK1 X035_pECIA2.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Mfsd10
Plasmid#120238PurposeExpresses V5-tagged Mfsd10 in lentiviral vectorDepositorAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2x/b
Plasmid#111569PurposeExpresses a plasma membrane calcium pump hPMCA2x/bDepositorInsertPlasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
TagsmCherryAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9-GFP S122D
Plasmid#114667PurposeExpresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tagDepositorInsertATG9
TagsGFPExpressionYeastMutationchanged serine 122 to aspartic acidPromoterATG9Available SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MIS12-FRB-FLAG
Plasmid#114040PurposeKinetochore localised FRB tagged with FLAGDepositorAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP
Plasmid#89062PurposeAAV-gRNA targeting the murine Apob geneDepositorInsertEmGFP
UseAAV and CRISPRExpressionMammalianPromoterChicken beta actin with partial CMV enhancerAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX3 (pcDNA3.1+)
Plasmid#75205PurposeMammalian expression of SLC24A3DepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR S155A
Plasmid#72702PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationS155APromoterubiquitinAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SMAD2
Plasmid#67095PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
WFS1 mRFP-N2 (891)
Plasmid#62018Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEg
Plasmid#61209PurposeFluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-GFP-BCP
TagsEGFPExpressionPlantPromoterMtBCP1Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MYLK4
Plasmid#39175PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE3 Xwnt-5A Full Length
Plasmid#16875DepositorInsertXwnt-5A (wnt5a.L Frog)
UseXenopus expressionAvailable SinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFoxd3IRESGFPtv
Plasmid#37266DepositorAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pEMS1231
Plasmid#29117PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable SinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Kif6_L (OZ537)
Plasmid#27214DepositorInsertZinc finger array targeting Kif6 (kif6 Zebrafish)
UseZebrafish targetingAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Kif6_R (OZ538)
Plasmid#27215DepositorInsertZinc finger array targeting Kif6 (kif6 Zebrafish)
UseZebrafish targetingAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSP64T XAR1 DNM1 (DM#102)
Plasmid#15042DepositorInsertXenopus activin receptor 1 (xstk2 Frog)
ExpressionBacterialMutationDominant negative mutantAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only