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169,450 results

Showing: 8321 - 8360 of 169450 results

  1. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  10. pD649-HAsp-FAS-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156541
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    FAS (FAS Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156541-plasmid-map-sequence-id-368509

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  19. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  23. p667-UBC-CDK12-V5_IDG-K

    Plasmid
    #135245
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5 (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135245-plasmid-map-sequence-id-268084

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  25. pNL[NLucP/IFNG/Hygro] Vector

    Plasmid
    #238626
    Purpose
    Express IFNG response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    IFNG (IFNG Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238626-plasmid-map-sequence-id-479511

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  33. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  37. pTIN-ura4

    Plasmid
    #161808
    Purpose
    A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe
    Depositor
    Stewart Shuman
    Type
    Empty backbone
    Expression
    Yeast
    Promoter
    Pho1
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    161808-plasmid-map-sequence-id-319459

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Showing: 8321 - 8360 of 169450 results