169,450 results
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Plasmid#214812PurposeMammalian expression of SpCas9 PE7 prime editorDepositorInsertPE7
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterCMVAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Hygro
Plasmid#164142PurposeRetroviral expression vector with hygromycin resistanceDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lenti-AsCpf1-Blast
Plasmid#84750PurposeExpresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertAsCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianPromoterEFS-NSAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEND-184_Cacnes_mCherry
Plasmid#225610PurposeReplicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsV5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP Donor
Plasmid#31475DepositorInsertSFFV d14GFP
UseLentiviralMutationDeleted 14 amino acids on the c terminus of eGFPAvailable SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PDPK1
Plasmid#23801DepositorInsertPDPK1 (PDPK1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR1
Plasmid#138192PurposeVirulence helperDepositorInsertvir gene cluster
UseVir gene expressionAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PKM2
Plasmid#23757DepositorInsertPKM2 (PKM Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH2_WT
Plasmid#81800PurposeGateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mouse av full length
Plasmid#217809PurposeFull length mouse integrin av expressionDepositorAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-STK38
Plasmid#23811DepositorInsertSTK38 (STK38 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-PA-BAX(mito)
Plasmid#228942PurposeStable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.DepositorUseRetroviralTagsEGFPExpressionMammalianMutationN-terminus and C-terminus are deleted. BAX(3-171)…Available SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
UAS-mNAMPT
Plasmid#187887PurposeGal4/UAS expression of mammalian NAMPTDepositorInsertmNAMPT
Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PCK2
Plasmid#23715DepositorInsertPCK2 (PCK2 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hPREP
Plasmid#179387PurposeExpress human prolyl oligopeptidase (tagged) in bacterial cellsDepositorAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HT028_FCMV_CheRiff_EGFP
Plasmid#178821PurposeExpresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tagDepositorInsertCheRIff_EGFP
UseLentiviralTagsEGFPExpressionMammalianPromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJS102
Plasmid#118281PurposeExpresses GFPmut2 with low cell-to-cell variation and lactose inductionDepositorInsertGFPmut2
ExpressionBacterialPromoterpLlacO-1Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI
Plasmid#159756PurposePiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backboneDepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianPromoterU6Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-SFXN1
Plasmid#110634PurposeRetroviral expression of Flag-SFXN1 in mammalian cellsDepositorInsertSFXN1 (SFXN1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)Available SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-STChRger2-TS-EYFP
Plasmid#129395PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianPromoterhSynAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT2-3xFLAG
Plasmid#163449Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)
Plasmid#197892PurposeCan be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInserthM4D/2A/GFP
UseAAVMutationN/APromoterEF1aAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-P2-SpCas9
Plasmid#232354PurposeThe plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.DepositorInsertsCas9
sacB
UseCRISPRPromoterpromoter P2 from S. marcescens HBQA7Available SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEdit
Plasmid#232355PurposeThe pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the tDepositorInsertsgRNA
UseCRISPRAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPIC9K_A2AR_316_V229C_R291A
Plasmid#246917PurposeExpress A2AR mutants in Pichia PastorisDepositorInsertAdenosine A2A receptor (ADORA2A Human)
Tags10xHis and alpha-factor secretion signal, FLAGExpressionYeastMutationtruncated to aa 1-316, V229C, R291APromoterAOX1Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
HRV-3C Protease-mCherry
Plasmid#243792PurposeEncodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertHRV-3C protease
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV.tetO.Nurr1
Plasmid#234848Purpose3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoterDepositorAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only