We narrowed to 15,100 results for: pET

1. pKHH030_sgCtrl#1

   Plasmid

   #174142

   Purpose

   Lentiviral vector expressing a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYEE

   Plasmid

   #159750

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-PEmax NG

   Plasmid

   #187894

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYUU

   Plasmid

   #159751

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1+FLAG-KrasnatG12D

   Plasmid

   #206842

   Purpose

   To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Aspartic Acid

   Promoter

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiCRISPRv2_sgCtrl#2

   Plasmid

   #174149

   Purpose

   Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   Control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-EF1a-DIO-FLPo-Myc

   Plasmid

   #124641

   Purpose

   AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   FLPo
   

   Use

   AAV and Cre/Lox

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-FDIO-ArchT-GFP

   Plasmid

   #124640

   Purpose

   AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   ArchT
   

   Use

   AAV; Flp/frt

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RRL.sin.cPPT.CMV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG494)

    Plasmid

    #139447

    Purpose

    Lentiviral vector to ectopically express human NCOA7 isoform 4 from CMV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTER RB1shRNA human

    Plasmid

    #66886

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

    Depositor

    Maria Alvarado-Kristensson

    Article

    Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

    Insert

    retinoblastoma 1 (RB1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-sgSmarca4#2/Cre

    Plasmid

    #173620

    Purpose

    Expresses a Smarca4-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Smarca4 (Smarca4 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCCL-PGK-SPdCas9-BFP-DNMT3A

    Plasmid

    #66819

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RRL.sin.cPPT.SFFV/Flag-NCOA7 variant 6.IRES-puro.WPRE (CG536)

    Plasmid

    #139443

    Purpose

    Lentiviral vector to ectopically express human NCOA7 isoform 4 from SFFV promoter

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-PE xCas9

    Plasmid

    #172652

    Purpose

    All-in-one prime editor piggyBac transposon (xCas9)

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTEE

    Plasmid

    #159748

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTUU

    Plasmid

    #159749

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

    Plasmid

    #99343

    Purpose

    Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

    Depositor

    Peter Wright

    Article

    Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

    Inserts

    Hif1a (776-826) (HIF1A Human)
    CBP (340-439)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKHH030_sgRB1#2

    Plasmid

    #174145

    Purpose

    Lentiviral vector expressing a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MigR1-AE9aP

    Plasmid

    #12434

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Nat Med. 2006 Aug . 12(8):945-9.

    Insert

    AML1-ETO9a as in t(8;21) patients (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AML1-ETO9a cDNA as in t(8;21) patients

    Promoter

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pCCL-PGK-SPdCas9-BFP-DNMT3B

    Plasmid

    #66820

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B, catalytic domain of DNMT3B
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid

    #191100

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    mScarlet-I-Cdt1 (30-120)
    

    Use

    AAV and Synthetic Biology

    Tags

    mScarlet-I fused to residues 30-120 of human Cdt1…

    Expression

    Mammalian

    Mutation

    Optimized to human codon usage

    Promoter

    CMV enhancer and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LentiGuide-Neo-NCOA7g1 (BB34)

    Plasmid

    #139452

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mcry1K31R HA tag

    Plasmid

    #110297

    Purpose

    mammalian expression of full length mouse CRY1 mutant K31R

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mcry1 K31R (Cry1 Mouse)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    31 Lysines are changed to Argenine

    Promoter

    CMV

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMMB67EH-dddAI-FLAG

    Plasmid

    #186560

    Purpose

    Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MigR1-AEtr

    Plasmid

    #12432

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    C-term truncated AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAFNF-PSDd1.2-GFP

    Plasmid

    #125581

    Purpose

    Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    PSDd1.2-GFP (Dlg4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-sgNcoa6#2/Cre

    Plasmid

    #173604

    Purpose

    Expresses a Ncoa6-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Ncoa6 (Ncoa6 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Lenti-sgNcoa6#1/Cre

    Plasmid

    #173603

    Purpose

    Expresses a Ncoa6-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Ncoa6 (Ncoa6 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

    Plasmid

    #99342

    Purpose

    Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

    Depositor

    Peter Wright

    Article

    Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

    Inserts

    STAT1 (710-750) (STAT1 Human)
    CBP (1764-1855)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV10-Flag-Ovtm

    Plasmid

    #51888

    Purpose

    expression vectore in mammalian cells

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mFbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ovtm (I364T)

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

35. NC4 pCDNA3.1 VENUS WTX (1-804)

    Plasmid

    #36959

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

    Plasmid

    #71219

    Purpose

    dCas9 fused to the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3B(E697A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPSV39-dddAI-FLAG

    Plasmid

    #186558

    Purpose

    Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBS10-ribE-dddAI-FLAG

    Plasmid

    #186562

    Purpose

    Regulated expression in Firmicutes of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pSpac(hy)

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMG035 MBP-Axin∆BCD-His

    Plasmid

    #154053

    Purpose

    Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. MBP-rPKL WT

    Plasmid

    #107156

    Purpose

    MBP-rPKL WT for production and purification of rat PKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCEE

    Plasmid

    #159746

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3.1/NDUFS2 _34-78/myc-His

    Plasmid

    #127496

    Purpose

    Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _34-78 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _34-78

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCS2-mkgDUX4mal*mqg*

    Plasmid

    #21174

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Promoter

    Available Since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

46. CMV-10-3xFLAG-LGP2-K651E

    Plasmid

    #58685

    Purpose

    Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

    Insert

    LGP2-K651E (DHX58 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    mutation to CTD, K651E

    Promoter

    CMV

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

47. Lenti CGIP + M-AAT target

    Plasmid

    #86008

    Purpose

    lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    M-AAT target
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V30M

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pNK091

    Plasmid

    #219769

    Purpose

    MoClo-compatible Level P vector carrying fungal bioluminescence system lacking luciferase gene

    Depositor

    Karen Sarkisyan

    Article

    Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

    Insert

    pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA-ATPT | p35S-nnCPH-ocsT | pFMV-nnH3H-nosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA…

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

    Plasmid

    #235266

    Purpose

    ComMAND EGFP-FMRP open-loop circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

    Plasmid

    #235267

    Purpose

    ComMAND EGFP-FMRP closed-loop circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge